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Garry Adams

Senior Professor

Curriculum Vitae

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Phone: (979) 845-9816

Mailstop: 4467

Department: VTPB

Photo of Adams, Garry

Education

  • Ph.D. Veterinary Pathology, Texas A&M University 1968
  • D.V.M. Veterinary Medicine (cum laude), Texas A&M University 1964
  • B.S. Animal Science (cum laude), Texas A&M University 1963
  • A.S. Biology, Tarleton State University 1961

Credentials

  • Diplomate American College of Veterinary Pathologists
  • Texas Veterinary Medical License: 2231

Scholarly Interests

I have long been intrigued with the biological interface between hosts and etiological agents, and the resulting patterns of morphological lesions, especially lesions caused by infectious disease agents. The morphologic, cytokine/chemokine, transcriptomic, proteomic and metabolomic patterns of host and pathogen responses provoke a series of fundamental questions, e.g. what is the molecular pathogenesis of these lesions?; how does the host genome manipulate the pathogen or vice versa?; what host and pathogen cellular pathways are perturbed and dysfunctional in the disease processes?; what is the cause of death?; can we apply genomic pathology (the convergence of ‘omics’ and morphology) and systems biology to more fully understand infection biology as the basis for improved prediction of host and pathogen mechanistic genes and pathways critical to health and clinical illness? In response to these questions, my research is focused on the: 1) investigation of the comparative molecular pathogenesis of zoonotic intracellular bacterial pathogens in natural target host animal models, particularly salmonellosis, brucellosis and mycobacterial diseases, 2) development of vaccines and host gene expression-based diagnostics for zoonotic and select agent caused diseases, and especially 3) development of in silico host:pathogen interactome predictive models based upon bi-directional in vivo host (bovine/murine) and Salmonella enterica Typhimurium interactions at the target organ interface, enteric Peyer’s patches. The vision to develop improved animal models for salmonellosis originated in the early 2000s in association with my collaborators, Drs. Andreas Bäumler and Renée Tsolis. From our very successful and continuing collaboration grew the idea to develop a computational infection biology model based on temporal neonatal calf in vivo RNA-Seq and microarray-based transcriptomic and proteomic profiling of the acute Salmonella, Brucella and Mycobacterium infection process. We expanded our research team by including Drs. Sara Lawhon, Kenneth Drake and Harold ‘Skip’ Garner, focusing on an envisioned systems biology analysis of both host and pathogen comprehensive transcriptomic and proteomic datasets derived from our in vivo biological model. We next computationally fused the datasets based on actual Salmonella proteomic data (performed by Dr. Mary Lipton at Pacific Northwest National Laboratory) and computationally predicted bovine host structural proteins to identify maximum likelihoods of host and pathogen protein:protein interactions as the basis for our preliminary in silico interactome model to predict mechanistic genes and linked perturbed cellular pathways. We then established in vitro phenotypes of S. Typhimurium deletion mutants (provided by Dr. Helene Andrews-Polymenis) of the predicted mechanistic genes for attachment, invasion or survival in murine RAW 264.7 macrophages to confirm almost half of the modeled predictions. Finally, over half of the model-predicted and in vitro-confirmed S. Typhimurium genes were validated to have in vivo phenotypes in our bovine ligated ileal loop model. We subsequently performed siRNA knockdowns in RAW 264.7 macrophages of predicted host proteins interacting with Salmonella proteins and validated in vitro phenotypes in almost half of the predicted host genes. By fusing higher quality genomic, transcriptomic, proteomic and metabolomic datasets derived from in vivo S. Typhimurium comparative infection models in mice, calves and non-human primates, we propose to further enhance the predictability and validation of in silico interactome model as a tool for the ultimate practice of “precision human medicine” as envisioned by the report of the National Academy of Sciences. As our publications document, we have also developed in silico interactome modeling tools for intracellular bacterial pathogens, Brucella melitensis and Mycobacterium avium ssp. paratuberculosis, all based on in vivo datasets. Our team has published 52 peer-reviewed manuscripts on salmonellosis using animal models, confirming our combined abilities to perform complex series of in vivo and in vitro experiments and developing high level in silico predictive models as we propose here. My long term role as co-developer of our in vivo based in silico interactome predictive model and personal collaborations with each of our team of investigators and core directors enables us to envision developing the in silico interactome predictive model for the biomedical research community.

Publications

Putting together a scientific team: collaborative science
L. Garry Adams
Trends in Microbiology.
Multicopy single-stranded DNA directs intestinal colonization of enteric pathogens
Elfenbein, Johanna R., Leigh A. Knodler, Ernesto S. Nakayasu, Charles Ansong, Heather M. Brewer, Lydia Bogomolnaya, L. Garry Adams, Michael McClelland, Joshua N. Adkins and Helene L. Andrews-Polymenis
PLoS Genetics.
Altered host immune responses to membrane vesicles from Salmonella and Gram-negative pathogens
Laughlin, Richard C., Megan Mickum, Kristina D Rowin, L. Garry Adams, and Robert Alaniz
Vaccine.
Pathogenesis and Immunobiology of Brucellosis: Review of Brucella-Host Interactions
de Figueiredo, Paul, Thomas A. Ficht, Allison Rice-Ficht, Carlos A. Rossetti and L. Garry Adams
American Journal of Pathology.
High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
Wu, J., Pugh, R., Laughlin, R. C., Andrews-Polymenis, H., McClelland, M., Bäumler, A. J., Adams, L. G.
Journal of Visualized Experiments.
Spatial segregation of virulence gene expression during acute enteric infection with Salmonella enterica serovar Typhimurium
Laughlin RC, Knodler LA, Barhoumi R, Payne HR, Wu J, Gomez G, Pugh R, Lawhon SD, Bäumler AJ, Steele-Mortimer O, Adams LG
mBio.
Human Brucellosis and Adverse Pregnancy Outcomes
Angela M. Arenas-Gamboa, Carlos A. Rossetti, Sankar P. Chaki, Daniel G. Garcia-Gonzalez, Leslie G. Adams, Thomas A. Ficht
Curt Trop Med Rep. DOI 10.1007/s40475-016-0092-0.
Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses
Köhler, Stephan, Nabil Hanna, Safia Ouahrani-Bettache, Kenneth L. Drake, L. Garry Adams, and Alessandra Occhialini
Stress and Environmental Control of Gene Expression in Bacteria.
Swine immunogenic properties of African Swine Fever vaccine candidates identified by reverse vaccinology
Lopera, Jaime, Jorge Osorio, Yongqun He, Sandesh Subramanya, L. Garry Adams, Richard C Laughlin, Waithaka Mwangi, John Neilan, David Brake, Thomas G Burrage, William Brown, Alfonso Clavijo, and Mangkey Bounpheng
Veterinary Immunology & Immunopathology. .
Structural and immunochemical aspects of Brucella abortus endotoxins.
Wu AM, MacKenzie NE, Adams G, Pugh R
Adv Exp Med Biol. 228: 551-76.
Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression.
Weeks JN, Galindo CL, Drake KL, Adams GL, Garner HR, Ficht TA
BMC Microbiol. 10: 167.
Effects of shipping and storage conditions of fecal samples on viability of Mycobacterium paratuberculosis.
Khare S, Adams LG, Osterstock J, Roussel A, David L
J Clin Microbiol. 46: 1561-2.
Diagnostic specificity of a real-time RT-PCR in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection.
Fosgate GT, Tavornpanich S, Hunter D, Pugh R, Sterle JA, Schumann KR, Eberling AJ, Beckham TR, Martin BM, Clarke NP, Adams LG
Vet Microbiol. 132: 158-64.
VirB12 is a serological marker of Brucella infection in experimental and natural hosts.
Rolán HG, den Hartigh AB, Kahl-McDonagh M, Ficht T, Adams LG, Tsolis RM
Clin Vaccine Immunol. 15: 208-14.
Modulation of the bovine trophoblastic innate immune response by Brucella abortus.
Carvalho Neta AV, Stynen AP, Paixão TA, Miranda KL, Silva FL, Roux CM, Tsolis RM, Everts RE, Lewin HA, Adams LG, Carvalho AF, Lage AP, Santos RL
Infect Immun. 76: 1897-907.
SdiA, an N-acylhomoserine lactone receptor, becomes active during the transit of Salmonella enterica through the gastrointestinal tract of turtles.
Smith JN, Dyszel JL, Soares JA, Ellermeier CD, Altier C, Lawhon SD, Adams LG, Konjufca V, Curtiss R 3rd, Slauch JM, Ahmer BM
PLoS One. 3.
Geostatistical analysis of biomarkers of genotoxicity in cattle, Bos taurus and Bos taurus x Bos indicus, sentinels near industrial facilities.
Bissett W Jr, Smith R, Adams LG, Field R, Moyer W, Phillips T, Scott HM, Thompson JA
Ecotoxicology. 18: 87-93.
Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil.
Paixão TA, Neta AV, Paiva NO, Reis JR, Barbosa MS, Serra CV, Silva RR, Beckham TR, Martin BM, Clarke NP, Adams LG, Santos RL
BMC Vet Res. 4: 53.
An evaluation of the health status of the Lavaca Bay, Texas ecosystem using Crassostrea virginica as the sentinel species.
Bissett W Jr, Smith R, Garry Adams L, Field R, Moyer W, Phillips T, Morgan Scott H, Wade T, Sweet S, Thompson JA
Mar Pollut Bull. 58: 280-6.
Salmonella enterica Typhimurium SipA induces CXC-chemokine expression through p38MAPK and JUN pathways.
Figueiredo JF, Lawhon SD, Gokulan K, Khare S, Raffatellu M, Tsolis RM, Bäumler AJ, McCormick BA, Adams LG
Microbes Infect. 11: 302-10.
Impairment of D-alanine biosynthesis in Mycobacterium smegmatis determines decreased intracellular survival in human macrophages.
Chacon O, Bermudez LE, Zinniel DK, Chahal HK, Fenton RJ, Feng Z, Hanford K, Adams LG, Barletta RG
Microbiology.
Contribution of flagellin pattern recognition to intestinal inflammation during Salmonella enterica serotype typhimurium infection.
Winter SE, Thiennimitr P, Nuccio SP, Haneda T, Winter MG, Wilson RP, Russell JM, Henry T, Tran QT, Lawhon SD, Gomez G, Bevins CL, Rüssmann H, Monack DM, Adams LG, Bäumler AJ
Infect Immun. 77: 1904-16.
Brucella melitensis global gene expression study provides novel information on growth phase-specific gene regulation with potential insights for understanding Brucella:host initial interactions.
Rossetti CA, Galindo CL, Lawhon SD, Garner HR, Adams LG
BMC Microbiol. 9: 81.
Early phase morphological lesions and transcriptional responses of bovine ileum infected with Mycobacterium avium subsp. paratuberculosis.
Khare S, Nunes JS, Figueiredo JF, Lawhon SD, Rossetti CA, Gull T, Rice-Ficht AC, Adams LG
Vet Pathol. 46: 717-28.
Life in the inflamed intestine, Salmonella style.
Santos RL, Raffatellu M, Bevins CL, Adams LG, Tükel C, Tsolis RM, Bäumler AJ
Trends Microbiol. 17: 498-506.
Morphologic and cytokine profile characterization of Salmonella enterica serovar typhimurium infection in calves with bovine leukocyte adhesion deficiency.
Nunes JS, Lawhon SD, Rossetti CA, Khare S, Figueiredo JF, Gull T, Burghardt RC, Bäumler AJ, Tsolis RM, Andrews-Polymenis HL, Adams LG
Vet Pathol. 47: 322-33.
The Salmonella enterica serotype Typhi Vi capsular antigen is expressed after the bacterium enters the ileal mucosa.
Tran QT, Gomez G, Khare S, Lawhon SD, Raffatellu M, Bäumler AJ, Ajithdoss D, Dhavala S, Adams LG
Infect Immun. 78: 527-35.
Vaccination with attenuated Salmonella enterica Dublin expressing E coli O157:H7 outer membrane protein Intimin induces transient reduction of fecal shedding of E coli O157:H7 in cattle.
Khare S, Alali W, Zhang S, Hunter D, Pugh R, Fang FC, Libby SJ, Adams LG
BMC Vet Res. 6: 35.
Bayesian Modeling of MPSS Data: Gene Expression Analysis of Bovine Salmonella Infection.
Dhavala SS, Datta S, Mallick BK, Carroll RJ, Khare S, Lawhon SD, Adams LG
J Am Stat Assoc. 105: 956-967.
Selective amplification of Brucella melitensis mRNA from a mixed host-pathogen total RNA.
Rossetti CA, Galindo CL, Garner HR, Adams LG
BMC Res Notes. 3: 244.
Gut inflammation provides a respiratory electron acceptor for Salmonella.
Winter SE, Thiennimitr P, Winter MG, Butler BP, Huseby DL, Crawford RW, Russell JM, Bevins CL, Adams LG, Tsolis RM, Roth JR, Bäumler AJ
Nature. 467: 426-9.
Amplified fragment length polymorphism reveals specific epigenetic distinctions between Mycobacterium avium subspecies paratuberculosis isolates of various isolation types.
O'Shea B, Khare S, Klein P, Roussel A, Adams LG, Ficht TA, Rice-Ficht AC
J Clin Microbiol. 49: 2222-9.
Enhancing the role of veterinary vaccines reducing zoonotic diseases of humans: linking systems biology with vaccine development.
Adams LG, Khare S, Lawhon SD, Rossetti CA, Lewin HA, Lipton MS, Turse JE, Wylie DC, Bai Y, Drake KL
Vaccine. 29: 7197-206.
Glennon, Elizabeth K. K., L. Garry Adams, Derrick Hicks, Katayoon Dehesh, Shirley Luckhart
Supplementation with abscisic acid reduces malaria disease severity and parasite transmission
American Journal of Tropical Medicine and Hygiene. 94: 1266-1275.
Systems Analysis of Early Host Gene Expression Provides Clues for Transient Mycobacterium avium spp avium vs. Persistent Mycobacterium avium spp paratuberculosis Intestinal Infections
Khare, Sangeeta, Kenneth L. Drake, Sara D. Lawhon, Jairo E. S. Nunes, Josely F. Figueiredo, Carlos A. Rossetti, Tamara Gull, Robin E. Everts, Harris A. Lewin, and L. Garry Adams
PLoS ONE. 11: e0161946.
Transcriptional profile of the intracellular pathogen Brucella melitensis following HeLa cells infection.
Rossetti CA, Galindo CL, Garner HR, Adams LG
Microb Pathog. 51: 338-44.
Multi-comparative systems biology analysis reveals time-course biosignatures of in vivo bovine pathway responses to B.melitensis, S.enterica Typhimurium and M.avium paratuberculosis.
Adams LG, Khare S, Lawhon SD, Rossetti CA, Lewin HA, Lipton MS, Turse JE, Wylie DC, Bai Y, Drake KL
BMC Proc.
Role of SPI-1 secreted effectors in acute bovine response to Salmonella enterica Serovar Typhimurium: a systems biology analysis approach.
Lawhon SD, Khare S, Rossetti CA, Everts RE, Galindo CL, Luciano SA, Figueiredo JF, Nunes JE, Gull T, Davidson GS, Drake KL, Garner HR, Lewin HA, Bäumler AJ, Adams LG
PLoS One. 6.
Improved variation calling via an iterative backbone remapping and local assembly method for bacterial genomes.
Tae H, Settlage RE, Shallom S, Bavarva JH, Preston D, Hawkins GN, Adams LG, Garner HR
Genomics. 100: 271-6.
Revised genome sequence of Brucella suis 1330.
Tae H, Shallom S, Settlage R, Preston D, Adams LG, Garner HR
J Bacteriol. 193: 6410.
Transcriptome analysis of HeLa cells response to Brucella melitensis infection: a molecular approach to understand the role of the mucosal epithelium in the onset of the Brucella pathogenesis.
Rossetti CA, Drake KL, Adams LG
Microbes Infect. 14: 756-67.
Complete genome sequence of Brucella suis VBI22, isolated from bovine milk.
Tae H, Shallom S, Settlage R, Hawkins GN, Adams LG, Garner HR
J Bacteriol. 194: 910.
Systems biology analysis of gene expression during in vivo Mycobacterium avium paratuberculosis enteric colonization reveals role for immune tolerance.
Khare S, Lawhon SD, Drake KL, Nunes JE, Figueiredo JF, Rossetti CA, Gull T, Everts RE, Lewin HA, Galindo CL, Garner HR, Adams LG
PLoS One. 7.
Comparison of genome diversity of Brucella spp. field isolates using Universal Bio-signature Detection Array and whole genome sequencing reveals limitations of current diagnostic methods.
Shallom SJ, Tae H, Sarmento L, Preston D, McIver L, Franck C, Dickerman A, Adams LG, Garner HR
Gene. 509: 142-8.
Salmonella enterica serovar Typhimurium-induced internalization and IL-8 expression in HeLa cells does not have a direct relationship with intracellular Ca(2+) levels.
Figueiredo JF, Barhoumi R, Raffatellu M, Lawhon SD, Rousseau B, Burghardt RC, Tsolis RM, Bäumler AJ, Adams LG
Microbes Infect. 11: 850-8.
A species independent universal bio-detection microarray for pathogen forensics and phylogenetic classification of unknown microorganisms.
Shallom SJ, Weeks JN, Galindo CL, McIver L, Sun Z, McCormick J, Adams LG, Garner HR
BMC Microbiol. 11: 132.
Comparative analysis of the early transcriptome of Brucella abortus--infected monocyte-derived macrophages from cattle naturally resistant or susceptible to brucellosis.
Rossetti CA, Galindo CL, Everts RE, Lewin HA, Garner HR, Adams LG
Res Vet Sci. 91: 40-51.
Correlative Gene Expression to Protective Seroconversation in Rift Valley Fever Vaccinates
Laughlin, R.L., Drake, K.L., Morrill, J.C., Wu, J., Adams, L.G
PLoS ONE. 11: e0147027.
Systems Biology Analysis of In Vivo Brucella melitensis Gene Expression during Colonization of bovine Peyer’s patch
Rossetti, C. A., Drake, K. L., Lawhon, S.L., Nunes, J., Gull, T., Khare, S., Everts, R., Lewin, H. and Adams, L. G.
Frontiers in Microbiology .


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