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Bovid DNA Testing

Services

Mitochondrial DNA (mtDNA) Introgression Typing

The mtDNA introgression assay is designed to detect the presence of domestic cattle mitochondrial DNA in bison or other wild bovids. The assay was developed in our laboratory and has been published (Ward et al. 1999).

Nuclear Introgression Typing

The nuclear introgression assay is based on a panel of 14 nuclear microsatellite markers designed to detect the presence of domestic cattle DNA (alleles) in bison. The assay was developed in our laboratory and has been published (Halbert et al. 2005). This assay is designed to detect low levels of domestic cattle DNA when large numbers of individuals (more than 100 from a given population) are evaluated. For a given individual from a population/herd, however, this assay will only detect low levels (0.5%) of introgression (domestic cattle DNA) about 6.8% of the time (see publication for more information). That is, we cannot PROVE that introgression does not exist for a given individual using this assay. However, if domestic cattle DNA (alleles) are detected for an individual bison, we have a very high level of confidence that the bison in question has domestic cattle ancestry.

Parentage Evaluation

The parentage assay is based on a panel of 11-15 nuclear microsatellite markers which are distinct from those using in the nuclear introgression panel. This assay was developed in our laboratory for use in bison and domestic cattle, and has been published (Schnabel et al. 2000). With this assay, we can determine the probability that a given individual (sire or dam) produced the offspring in question. Pricing differs based on whether a sample from the known dam is provided (preferred, but not required).

Pricing 

  • Pricing can be negotiated downward for a large number of samples and FTA cards.
Standard services # samples Price Comment
mtDNA introgression typing (bison only) 1 to 99 $12 per sample
mtDNA introgression typing (bison only) 100 to 999

$8 per hair, $7 per FTAsample

mtDNA and nuclear introgression typing (bison only) 1 to 99 $35 per sample
mtDNA and nuclear introgression typing (bison only) 100 to 999 $25 per sample
nuclear introgression typing (bison only) 1 to 99 $35 per sample
nuclear introgression typing (bison only) 100 to 999 $25 per sample
parentage typing/sire verification (domestic cattle or bison) any $45 per 3-way offspring comparison Customer provides samples from offspring, known dam, and potential sire (for each potential sire, an additional $25 is charged). We will provide marker genotypes and an analysis of whether the potential sire is excluded or cannot be excluded.
blind parentage analysis (domestic cattle or bison) 1 to 99 $35 per sample Customer provides samples from offspring and all possible dams and sires; charge is for each sample used in the comparison (all offspring + all sires + all dams). Pricing also applies in cases where sample from dam is NOT provided.
blind parentage analysis (domestic cattle or bison) 100+ $25 per sample Customer provides samples from offspring and all possible dams and sires; charge is for each sample used in the comparison (all offspring + all sires + all dams). Pricing also applies in cases where sample from dam is NOT provided.
all assays (mtDNA and nuclear introgression PLUS parentage typing/sire verification) 1 to 99 $45 per sample For whole herd blind parentage analysis (neither parent known), additional costs will apply.
all assays (mtDNA and nuclear introgression PLUS parentage typing/sire verification) 100+ $40 per sample For whole herd blind parentage analysis (neither parent known), additional costs will apply.