Fragment Analysis Services
Sample of microsatellite fragments (alleles) produced with
fluorescent-tagged primers on ABI 3130xl (6 multiplexed markers
shown, dye set DS-31)
Services and Pricing
| Client provides size standard |
$1.50 |
Requestor provides PCR products and size standard |
|
| We provide size standard |
$2.00 |
Requestor provides PCR products & we provide size
standard |
Mapmarker 400-ROX |
| 96-well plate ready-to-run |
$100 |
Requestor provides 96-well plate of products in formamide ready
to load with electronic sample sheet |
Sample sheet template (Instructions) |
Forms and Instructions
Software and Instrumentation
ABI 3130xl Genetic Analyzer
- All fragments are separated and detected on an ABI 3130xl
Genetic Analyzer
- 96- or 384-well plate format
- 16 samples per injection (run times vary from 20-40 minutes
depending on desired read length)
- 36-cm array with POP7 polymer capable of analyzing fragments up
to 1000-bp
- Sizing standards may be purchased through Applied
Biosystems (GeneScan) or BioVentures
(MapMarker)
- Dye sets currently supported:
| Dye Set |
Filter Set |
Size Standard Dye |
Fragment Detection Dyes |
| DS-02 |
E5 (SNaPshot) |
LIZ |
dR110, dR6G, dTAMRA, dROX |
| DS-30 |
D |
ROX |
6-FAM, HEX, NED |
| DS-31 |
D |
ROX |
6-FAM, VIC, NED |
| DS-33 |
G5 |
LIZ |
6-FAM, VIC, NED, PET |
Analysis/Viewing Software
A NanoDrop ND-1000 Spectrophotomer
- A NanoDrop ND-1000 Spectrophotomer is available for customer
use to quantify DNA, primers, RNA, etc.