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Sequence-Based Microorganism Identification Services

Sequencing screenshot

Sequence-based identification using the MicroSeq Microbial Identification System (Applied Biosystems)

Please Note: We can only accept bacterial and fungal DNA at this time.
* * NO LIVE CULTURES * *

Turnaround Time

  • Samples received by noon will be processed the same day with results by the following morning.
  • Samples received after noon will be processed the next day with results by the following morning.
  • Samples are not processed on Wednesdays. Samples received on Wednesday will be processed on Thursday.

Note: Results will be sent via email.

This project was supported by Texas A&M AgriLife , the Department of Veterinary Pathobiology in the College of Veterinary Medicine, and the Texas Veterinary Medical Diagnostic Laboratory .


Services and Pricing

  • 16S 500-bp Fragment Sequencing - $50.00
    • Primary method of identification
    • In most cases, 500-bp sequencing is sufficient to identify to species-level
    • Results within 24-hours of sample receipt
  • 16S Full Gene Sequencing - $100.00
    • Used to further resolve identity if 500-bp sequence yields ambiguous results
    • Can also be requested upfront (500-bp sequencing is not required to be performed first)
  • Fungal Sequencing - $50.00
    • Sequences D2 region of the large-subunit (LSU) rRNA gene
    • Results within 24-hours of sample receipt

Forms and Instructions

Process

  • Check DNA quantity
    • NanoDrop ND-1000 Spectrophotometer
  • Amplify target region of gene
    • 16S universal primers or D2 LSU universal primers
  • Sequence PCR products
    • BigDye Terminator v1.1 chemistry
  • Analyze data using MicroSeq ID software and GenBank
    • Comparison against validated MicroSeq library and published GenBank submissions
  • Produce report containing results of data analysis