Fecal ELISA for Clostridium difficile toxin A and/or B
For these tests we will require up
to 1 gram of fresh feces. Samples should be refrigerated shortly
after collection and shipped overnight cooled with ice packs
us concerning our shipping program).
a gram-positive anaerobic, spore-forming rod is a major cause of
pseudomembranous colitis in humans and has also been associated
with diarrhea in dogs and cats. The virulence of C.
difficile is associated with the presence of genes that code
for various toxins, most notably toxin A (an enterotoxin) and toxin
B (a cytotoxin). Isolation rates for C. difficile
range between 0-40% in different studies, but isolation frequencies
have been similar between diarrheic and non-diarrheic dogs.
Therefore, the significance of detecting C. difficile
organisms in fecal samples from dogs with diarrhea remains unclear.
Approximately 50% of C. difficile organisms harbor the
genes for toxins A and/or B.
However, the presence of the gene
does not prove the synthesis and release of these toxins. Thus,
currently the most accurate method for diagnosis of C.
difficile-associated diarrhea is the detection of toxins A
and B by ELISA.
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