Digital Imaging

Zeiss Digital Imaging Workstation

• Zeiss Axioplan Microscope with motorized z-stage and DAPI, FITC, Rhodamine, and Texas Red fluorescence filters
• Ziess Axiophot supporting a Zeiss Axiocam capable of color image capture at 3900 x 3090 pixel resolution
• Two film cameras
• Pentium 4 computer
• Epson Expression 636 scanner
• Kodak 8650 PS "photographic quality" thermal dye printer

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Olympus Digital Imaging

• Inverted Olympus IX70 Microscope with motorized xyz-stage
• ORCA-ER B/W CCD digital camera
• Pentium IV computer with 512 MB RAM and 800 MHZ
• Simple PCI software for Data acquisition and analysis

Zeiss 510 META NLO Multiphoton Microscope

• Zeiss Axiovert 200 MOT microscope
• Coherent Chameleon pulsed infrared multiphoton tunable from 720-950 nm
• Laser lines at 458, 477, 488, 514, 543 and 633 nm
• Z-stack collection, spectral emission profiling and seperation
• Multi-time, Physiology, FRET, FRAP software
• Two confocal channels, one spectral detection channel (META), two channels non-descanned detection, and one transmitted light channel
• Objectives:
  • Plan-Neofluar 10x/0.3 NA
  • Plan-Apochromat 20x / 0.8 NA
  • Plan-Neofluar 40x / 0.85 NA
  • Plan-Neofluar 40x / 1.30 NA Oil
  • Plan Apochromat 63x / 1.4 NA Oil
  • C-Apochromat 40x / 1.2 NA Water

Bio-Rad Radiance 2000 MP

• Equipped with Tsunami-Millenia Laser: Wavelength range 690-1047nm
• Equipped with Argon Krypton Laser (488nm and 568nm) and Red diode laser (637nm)
• Capable of simultaneous, three-wavelength excitation
• Three photomultiplier tubes and an array of detection filter sets
• Equipped with Nikon Eclipse TE 300 Inverted Microscope.
• Laser sharp 2000 software

Stallion Digital Imaging Workstation

• Xenon fluorescent light source, 300 W with rapid switching (<2 msec) between excitation
  wavelengths
• Synchronization hardware (TTL-based)
• Shutter for transmitted light (25 mm)
• Incubator with CO2 and temperature Control
• 2 x CoolSnap HQ Camera with external ventilation
• Stallion software
• Ratio/FRET Software Module
• Siemens/Fujitsu 20 A computer
• Antivibration table

Zeiss TIRF

• Zeiss Axio Observer Z1 Microscope fully motorized with DAPI, FITC, and Rhodamine, and Texas Red fluorescence filters
• Two cameras: High resolution AxioCam MRm and Roper S/W PVCAM
• Laser Lines: 405nm, 488nm, 514 nm, 561 nm
• Incubator for temperature and CO₂ control
• AxioVision 4 Software

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FEI Transmission Electron Microscope

• High resolution TEM with top entry stage
• High-Voltage range: 40 to 100kV in steps of 10kV
• Intensity zoom: allows for constant screen brightness at different magnifications
• Intensity limit: prevents electron beam intensity overload on sample
• Magnification: 25 - 200000 x
• Automatic saving of full exposure sequence
• Integrated Dual Pentium PC with Windows operating system

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