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Imaging Capabilities

The IAL facility has a range of microscopes and related systems that offer a wide array of capabilities. Some of the capabilities are listed below. If you have questions as to the best way to proceed with your project please contact us.

Fluorescence imaging of a range of samples

  • Different modalities for different specimens - widefield, deconvolution, laser scanning confocal, multiphoton, TIRF, super resolution
  • Filters/excitation for numerous fluorophores
  • Laser lines available: 405, 458, 477, 488, 514, 543, 561, 594, 633, 647 and 720-950 nm (pulsed IR)

Transmitted light imaging

  • DIC, phase contrast, brightfield, darkfield, polarized light
  • Color imaging for IHC, special stains for histology, etc.

Live-cell imaging

  • Temperature, humidity and CO2 regulation on widefield fluorescence, TIRF, confocal, multipohton and super resolution microscopes
  • High-sensitivity EM-CCD camera (quantum efficiency up to 92%) on widefield and TIRF systems
  • Time lapse

FRAP, FLIP, Photoactivation/conversion

  • Measure protein dynamics in living cells
  • Point and line scanning confocal, convenient for defined regions with slower processes
  • Point activation/bleaching

FRET, FLIM

  • Sensitized emission and acceptor photobleaching
  • Possible on confocal and widefield systems
  • Ratiometric CFP/YFP FRET reporter imaging
  • Lifetime measurements on frequency domain FLIM system

Image processing, analysis, quantification

  • Measurement of intensity, size, number, etc.
  • Object tracking
  • 3D measurement
  • Deconvolution
  • Pattern recognition
  • Statistical analysis using SAS, Minitab, Prism
  • Additional algorithms may be developed using Labview and/or Matlab

Transmission electron microscopy

  • Fixation, tissue processing and resin embedding
  • Ultra-microtomy and post-staining of sections
  • Immunogold labeling
  • Negative stain preparations
  • Image interpretation and analysis

Please contact us if there are other techniques or capabilities that you may need as it may be possible to add these to our facilities and services.