Lightsheet Fluorescence Microscopy Demo June 4-8, 2018
Posted May 31, 2018
We’re are pleased to be hosting the Phase View
ALPHA3 Light Sheet Microscope in the
College of Veterinary Medicine & Biomedical Sciences Image
Please join us on June 4 at 1:00 pm for a short seminar in Room
107D of the Veterinary Medical Administration Building.
The demos will be starting from 9:00 am June 5 through June
8. Three users or groups of users per day will be scheduled
during that week.
The light sheet system can host small live samples such as
zebrafish embryo, drosophila larvae, C. elegans, or fixed samples
such as tissue slices, spheroids, thick organoids slices or entire
organoids, an entire mouse kidney or an entire small mouse brain
(up to 10 x 10 x 10 mm).
Of course, large samples have to be cleared in advance of
imaging. For cell cultures, cells will need to be plated on a
12 mm round coverslip.
Protocols for clearing include iDISCO which can be easily imaged
through water dipping lenses. Other protocols can be used as
well like CLARITY or CUBIC but it is better to image such samples
with dedicated objectives (like the CLARITY objectives from
Olympus) so please let us know ASAP if you plan to use such
protocols as we will need to request these specific objectives.
- If you have a sample their own sample you should schedule
a slot of 3 hours so we have time for mounting the sample, playing
with it and imaging. Please describe details about your samples in
the comments (i.e., what it is, the size of the sample, in which
media, which laser lines needed, etc.
- If you want to do an overnight imaging session, please schedule
a slot quite late in the afternoon (e.g., 4-7 pm) .
- If you do not have your own samples, then 1.5 hour should be
- We will attempt to schedule in 3 hr blocks which can be split
into 1.5 hr blocks if you don’t have your own samples (9am-12pm /
1pm-4pm / 4pm-7pm).
A Google Calendar is provided here for scheduling the
If for any reason you cannot schedule a time through the
provided link, Please contact me and I will be happy to schedule
you a demo ttime.
Rola Barhoumi Mouneimne, Ph.D.
Research Professor & Associate Director,
Image Analysis Laboratory
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