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Lightsheet Fluorescence Microscopy Demo June 4-8, 2018

Posted May 31, 2018

We’re are pleased to be hosting the Phase View  ALPHA3 Light Sheet Microscope in the College of Veterinary Medicine & Biomedical Sciences Image Analysis Laboratory.

Please join us on June 4 at 1:00 pm for a short seminar in Room 107D of the Veterinary Medical Administration Building.   The demos will be starting from 9:00 am June 5 through June 8.  Three users or groups of users per day will be scheduled during that week.


The light sheet system can host small live samples such as zebrafish embryo, drosophila larvae, C. elegans, or fixed samples such as tissue slices, spheroids, thick organoids slices or entire organoids, an entire mouse kidney or an entire small mouse brain (up to 10 x 10 x 10 mm).

Of course, large samples have to be cleared in advance of imaging.  For cell cultures, cells will need to be plated on a 12 mm round coverslip.

Protocols for clearing include iDISCO which can be easily imaged through water dipping lenses. Other protocols can  be used as well like CLARITY or CUBIC but it is better to image such samples with dedicated  objectives (like the CLARITY objectives from Olympus) so please let us know ASAP if you plan  to use such protocols as we will need to request these specific objectives.

  • If you  have a sample their own sample you should schedule a slot of 3 hours so we have time for mounting the sample, playing with it and imaging. Please describe details about your samples in the comments (i.e., what it is, the size of the sample, in which media, which laser lines needed, etc.
  • If you want to do an overnight imaging session, please schedule a slot quite late in the afternoon (e.g., 4-7 pm) .
  • If you do not have your own samples, then 1.5 hour should be enough.
  • We will attempt to schedule in 3 hr blocks which can be split into 1.5 hr blocks if you don’t have your own samples (9am-12pm / 1pm-4pm / 4pm-7pm).

A Google Calendar is provided here for scheduling the instrument: :

If for any reason you cannot schedule a time through the provided link, Please contact me and I will be happy to schedule you a demo ttime.


Rola Barhoumi Mouneimne, Ph.D.

Research Professor & Associate Director,

Image Analysis Laboratory


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