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The College of Veterinary Medicine & Biomedical Sciences (CVM) Image Analysis Laboratory (IAL) supports the microscopy needs of basic and clinical scientists and their trainees carry out research to improve the health of animals, humans and the environment that is consistent with the One Health Initiative of the Texas A&M University System. Through this initiative, the CVM IAL engages in interdisciplinary collaborations with numerous colleges and interdisciplinary programs. It also serves as an Advanced Imaging Facility Core for the interdisciplinary NIEHS-supported Center for Translational Environmental Health Research (CTEHR). The CTEHR, a National Center of Excellence in Environmental Health Science is a joint initiative between Texas A&M University, Texas A&M AgriLife Research, Baylor College of Medicine, and the University of Houston.

Listed below are some of the scholarly contributions from users of the Laboratory that includes journal covers featuring images acquired at the IAL and representative recent publications from IAL users.

We appreciate acknowledgement and notification of publications that involved the use of IAL so we can demonstrate productivity for funding of grant applications. We ask users of the laboratory to email us with publications that they would like to be listed.


journals cover

Selected Publications

Chaki SP, Barhoumi R, Rivera GM. Actin remodeling by Nck regulates endothelial lumen formation. Mol Biol Cell. 2015 Sep 1;26(17):3047-60. doi: 10.1091/mbc.E15-06-0338. Epub 2015 Jul 8. PubMed PMID: 26157164; PubMed Central PMCID: PMC4551318.

Banu SK, Stanley JA, Sivakumar KK, Arosh JA, Barhoumi R, Burghardt RC. Identifying a novel role for X-prolyl aminopeptidase (Xpnpep) 2 in CrVI-induced adverse effects on germ cell nest breakdown and follicle development in rats. Biol Reprod. 2015 Mar;92(3):67. doi: 10.1095/biolreprod.114.125708. Epub 2015 Jan 7. PubMed PMID: 25568306; PubMed Central PMCID: PMC4358023.

Stanley JA, Arosh JA, Burghardt RC, Banu SK. A fetal whole ovarian culture model for the evaluation of CrVI-induced developmental toxicity during germ cell nest breakdown. Toxicol Appl Pharmacol. 2015 Sep 5. pii: S0041-008X(15)30076-4. doi: 10.1016/j.taap.2015.09.002. [Epub ahead of print] PubMed PMID: 26348139.

Arosh JA, Lee J, Balasubbramanian D, Stanley JA, Long CR, Meagher MW, Osteen KG, Bruner-Tran KL, Burghardt RC, Starzinski-Powitz A, Banu SK. Molecular and preclinical basis to inhibit PGE2 receptors EP2 and EP4 as a novel nonsteroidal therapy for endometriosis. Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):9716-21. doi: 10.1073/pnas.1507931112. Epub 2015 Jul 21. PubMed PMID: 26199416; PubMed Central PMCID: PMC4534219.

Ma X, Hou YQ, Dahanayaka S, Satterfield MC, Burghardt RC, Bazer FW, Wu G. Technical note: Isolation and characterization of ovine brown adipocyte precursor cells. J Anim Sci. 2015 May;93(5):2094-9. doi: 10.2527/jas.2014-8728. PubMed PMID: 26020305.

Wang X, Burghardt RC, Romero JJ, Hansen TR, Wu G, Bazer FW. Functional roles of arginine during the peri-implantation period of pregnancy. III. Arginine stimulates proliferation and interferon tau production by ovine trophectoderm cells via nitric oxide and polyamine-TSC2-MTOR signaling pathways. Biol Reprod. 2015 Mar;92(3):75. doi: 10.1095/biolreprod.114.125989. Epub 2015 Feb 4. PubMed PMID: 25653279.

Barhoumi R, Mouneimne Y, Chapkin RS, Burghardt RC. Effects of Fatty Acids on Benzo[a]pyrene Uptake and Metabolism in Human Lung Adenocarcinoma A549 Cells. PLoS One. 2014 Mar 20;9(3):e90908. PubMed PMID: 24651647; PubMed Central PMCID: PMC3961214.

Peña B, Barhoumi R, Burghardt RC, Turro C, Dunbar KR. Confocal fluorescence microscopy studies of a fluorophore-labeled dirhodium compound: visualizing metal-metal bonded molecules in lung cancer (A549) cells. J Am Chem Soc. 2014 May 22. [Epub ahead of print]

Jutooru I, Guthrie AS, Chadalapaka G, Pathi S, Kim K, Burghardt R, Jin UH, Safe S. Mechanism of action of phenethylisothiocyanate and other ROS-inducing anticancer agents. Mol Cell Biol. 2014 Apr 14. [Epub ahead of print]

Laughlin RC, Knodler LA, Barhoumi R, Payne HR, Wu J, Gomez G, Pugh R, Lawhon SD, Bäumler AJ, Steele-Mortimer O, Adams LG. Spatial segregation of virulence gene expression during acute enteric infection with Salmonella enterica serovar Typhimurium. MBio. 2014 Feb 4;5(1):e00946-13. PubMed PMID: 24496791; PubMed Central PMCID: PMC3950517.

Kim W, Barhoumi R, McMurray DN, Chapkin RS. Dietary fish oil and DHA down-regulate antigen-activated CD4+ T-cells while promoting the formation of liquid-ordered mesodomains. Br J Nutr. 2014 Jan 28;111(2):254-60PubMed PMID: 23962659.

Li X, Bazer FW, Johnson GA, Burghardt RC, Frank JW, Dai Z, Wang J, Wu Z, Shinzato I, Wu G. Dietary supplementation with L-arginine between days 14 and 25 of gestation enhances embryonic development and survival in gilts. Amino Acids. 2014 Feb;46(2):375-84. PubMed PMID: 24337831.

Sivakumar KK, Stanley JA, Arosh JA, Pepling ME, Burghardt RC, Banu SK. Prenatal exposure to chromium induces early reproductive senescence by increasing germ cell apoptosis and advancing germ cell cyst breakdown in the F1 offspring. Dev Biol. 2014 Apr 1;388(1):22-34. PubMed PMID: 24530425.

Wang X, Frank JW, Little DR, Dunlap KA, Carey Satterfield M, Burghardt RC,Hansen TR, Wu G, Bazer FW. Functional role of arginine during the peri-implantation period of pregnancy. I. Consequences of loss of function of arginine transporter SLC7A1 mRNA in ovine conceptus trophectoderm. FASEB J. 2014 Mar 13. PubMed PMID: 24627544.

Li F, Yu X, Szynkarski CK, Meng C, Zhou B, Barhoumi R, White RE, Heaps CL, Stallone JN, Han G. Activation of GPER Induces Differentiation and Inhibition of Coronary Artery Smooth Muscle Cell Proliferation. PLoS One. 2013 Jun 19;8(6):e64771. PubMed PMID: 23840305; PubMed Central PMCID: PMC3686788.

Chaki SP, Barhoumi R, Berginski ME, Sreenivasappa H, Trache A, Gomez SM, Rivera GM. Nck enables directional cell migration through the coordination of polarized membrane protrusion with adhesion dynamics. J Cell Sci. 2013 Apr 1;126(Pt 7):1637-49. PubMed PMID: 23444376.

Streifel KM, Miller J, Mouneimne R, Tjalkens RB. Manganese inhibits ATP-induced calcium entry through the transient receptor potential channel TRPC3 in astrocytes. Neurotoxicology. 2013 Jan;34:160-6. PubMed PMID: 23131343; PubMed Central PMCID: PMC3557543.

Oelusarz A, Nichols LA, Grunz-Borgmann EA, Chen G, Akintola AD, Catania JM, Burghardt RC, Trzeciakowski JP, Parrish AR. Overexpression of MMP-7 increases collagen 1A2 in the aging kidney. Physiol Rep. 2013 Oct;1(5). pii: e00090.

Kim K, Jutooru I, Chadalapaka G, Johnson G, Frank J, Burghardt R, Kim S, Safe S. HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer. Oncogene. 2013 Mar 28;32(13):1616-25.

Baker LK, Gillis DC, Sharma S, Ambrus A, Herrmann H, Conover G. Nebulin binding impedes mutant desmin filament assembly. Molecular Biology of the Cell. 2013;24:1918-32. PubMed PMID: 23615443.

Storey SM, McIntosh AL, Huang H, Martin GG, Landrock KK, Landrock D, Payne HR, Kier AB, Schroeder F. Loss of Intracellular Lipid Binding Proteins Differentially Impacts Saturated Fatty Acid Uptake and Nuclear Targeting in Mouse Hepatocyes. American journal of physiology Gastrointestinal and liver physiology. 2012;303:G837-G50. PubMed PMID: 22859366; PubMed Central PMCID: PMC3469595.

Storey SM, McIntosh AL, Huang H, Landrock KK, Martin GG, Landrock D, Payne HR, Atshaves BP, Kier AB, Schroeder F. Intracellular Cholesterol Binding Proteins Enhance HDL-mediated Cholesterol Uptake in Cultured Primary Mouse Hepatocytes. American journal of physiology Gastrointestinal and liver physiology. 2012. Epub Jan 12, 2012. PubMed PMID: 22241858; PubMed Central PMCID: PMC3355564.

Turk HF, Barhoumi R, Chapkin RS. Alteration of EGFR spatiotemporal dynamics suppresses signal transduction. PLoS One. 2012;7(6):e39682. PubMed PMID: 22761867; PubMed Central PMCID: PMC3384615.

Steelman AJ, Thompson JP, Li J. Demyelination and remyelination in anatomically distinct regions of the corpus callosum following cuprizone intoxication. Neuroscience Research. 2012;72:32-42. PubMed PMID: 22015947.

Stoica G, Lungu G, Bjorklund NL, Taglialatela G, Zhang X, Chiu V, Hill HH, Schenk JO, Murray I. Potential role of α-synuclein in neurodegeneration: studies in a rat animal model. J Neurochem. 2012;122:812-22. PubMed PMID: 22639889.

Hou TY, Monk JM, Fan YY, Barhoumi R, Chen YQ, Rivera GM, McMurray DN, Chapkin RS. n-3 polyunsaturated fatty acids suppress phosphatidylinositol 4,5-bisphosphate-dependent actin remodeling during CD4+ T-cell activation. Biochem J. 2012 Apr 1;443(1):27-37. PubMed PMID: 22250985; PubMed Central PMCID: PMC3814172.

Kamkaew A, Barhoumi R, Burghardt RC, Burgess K. Cationic polyfluorenes for intracellular delivery of proteins. Org Biomol Chem. 2011 Oct 7;9(19):6513-8. PubMed PMID: 21845277; PubMed Central PMCID: PMC3433840.

Tiffany-Castiglioni E, Qian Y, Barhoumi R, Zheng Y. Determination of metal interactions with the chaperone Hspa5 in human astrocytoma cells and rat astrocyte primary cultures. Methods Mol Biol. 2011;758:29-48. PubMed PMID: 21815057.

Ibrahim A, El-Meligy A, Lungu G, Fetaih H, Dessouki A, Stoica G, Barhoumi R. Curcumin induces apoptosis in a murine mammary gland adenocarcinoma cell line through the mitochondrial pathway. Eur J Pharmacol. 2011 Oct 1;668(1-2):127-32. PubMed PMID: 21762689.

Kolar S, Barhoumi R, Jones CK, Wesley J, Lupton JR, Fan YY, Chapkin RS. Interactive effects of fatty acid and butyrate-induced mitochondrial Ca²⁺ loading and apoptosis in colonocytes. Cancer. 2011 Dec 1;117(23):5294-303. PubMed PMID: 21563175; PubMed Central PMCID: PMC3156959.

Jose J, Loudet A, Ueno Y, Wu L, Chen HY, Son DH, Barhoumi R, Burghardt R, Burgess K. Energy transfer cassettes in silica nanoparticles target intracellular organelles. Org Biomol Chem. 2011 May 21;9(10):3871-7. PubMed PMID: 21455504.

Barhoumi R, Mouneimne Y, Ramos E, Morisseau C, Hammock BD, Safe S, Parrish AR, Burghardt RC. Multiphoton spectral analysis of benzo[a]pyrene uptake and metabolism in a rat liver cell line. Toxicol Appl Pharmacol. 2011 May 15;253(1):45-56. PubMed PMID: 21420996; PubMed Central PMCID: PMC3081912.

Storey SM, Atshaves BP, McIntosh AL, Landrock D, Martin GG, Huang H, Payne HR, Johnson JD, Macfarlane RD, Kier AB, Schroeder F. Effect of sterol carrier protein-2 gene ablation on HDL-mediated cholesterol efflux from cultured primary mouse hepatocytes. American journal of physiology Gastrointestinal and liver physiology. 2010;299:G244-G54. PubMed PMID: 20395534; PubMed Central PMCID: PMC2904118.

Porter BF, Ridpath JF, Calise DV, Payne HR, Janke JJ, Baxter DG, Edwards JF. Hypomyelination associated with bovine viral diarrhea virus type 2 infection in a longhorn calf. Vet Pathol. 2010;47(4):658-63. PubMed PMID: 20448278.

Hoppes S, Gray PL, Payne S, Shivaprasad HL, Tizard I. The Isolation, pathogenesis, diagnosis, transmission and control of Avian Bornavirus and proventricular dilatation disease. Veterinary Clinics of North America Exotic Animal Practice. 2010;13:495-508. PubMed PMID: 20682432.

Maheshwari M, Roberts JK, DeSutter B, Duong K, Tingling J, Fawver JN, Schall HE, Kahle M, Murray IVJ. Hydralazine modifies Aß fibril formation and prevents modification by lipids in vitro. Biochemistry. 2010;49:10371-80. PubMed PMID: 21058733.

Ibrahim A, El-Meligy A, Fetaih H, Dessouki A, Stoica G, Barhoumi R. Effect of curcumin and Meriva on the lung metastasis of murine mammary gland adenocarcinoma. In Vivo. 2010 Jul-Aug;24(4):401-8. PubMed PMID: 20668306.

Vanderlaag K, Su Y, Frankel AE, Burghardt RC, Barhoumi R, Chadalapaka G, Jutooru I, Safe S. 1,1-Bis(3'-indolyl)-1-(p-substituted phenyl)methanes induce autophagic cell death in estrogen receptor negative breast cancer. BMC Cancer. 2010 Dec 3;10:669. PubMed PMID: 21129193; PubMed Central PMCID: PMC3003661.