Ondonsterpoort Animal Research Center Externship
The staff of Ondonsterpoort Veterinary Institute were wonderful
hosts and provided us with a diverse and comprehensive view of
diagnosis and management of disease. They also provided us housing
on the beautiful campus!
We spent the first week with in the Tuberculosis
Lab. The lab functions mainly as a diagnostic laboratory for South
Africa as well as many other neighboring African countries; they
preform post mortem tests as well as screening tests for movement
of animals especially Cape Buffalo. In addition to standard culture
with microscopic identification, they preform PCR in order to
identify the species of TB isolated (M. bovis, M. tuberculosis, M.
bovis BCG, M. microti, M. canettii, M. pinnipedi, M. africum, M.
caprae, M. mungi, and M. sucricattae). They start with a screening
PCR for the most common isolate M. bovis and then have three other
PCR tests to differentiate the others.
The research component of the laboratory focuses on the
epidemiology of TB on the African continent. They will take each
sample and identify the strain of TB within the species in order to
better understand the transmission of TB between the different
species of wildlife and domestic livestock.
On our free weekend, we went to Dinokeng Game Reserve on the
suggestion of our hosts. There we were able to see some of the
wildlife species whose samples we were processing at the laboratory
such as impala, nyala, Cape buffalo, warthog, and white rhino. We
were also able to speak to locals who deal with the implications of
the livestock/wildlife interface every day. Johan, who is a
microlight pilot on the reserve, struggles with abortions in his
goat herd likely due to their contact with wild game on the reserve
who harbor diseases such as Q Fever and Brucellosis.
The second week, we spent in the Transboundary Disease
Laboratories. This facility houses mainly the African Swine Fever
(ASF) and Foot and Mouth Disease (FMD) diagnostic and research
laboratories. We participated in the diagnostic component which
consists of mainly serology, PCR, and virus isolation. There was an
ASF outbreak currently going on in the Free State of South Africa
during our stay and as the OIE reference laboratory for ASF, the
lab was currently receiving all samples for confirmation.
This is a BSL 3 laboratory with a shower out policy
so we gained experience with measures used to maintain biosecurity
when working with these highly virulent pathogens. During our stay,
there was also a Rift Valley Fever vaccine challenge going on.
Sadly, because we are not previously exposed or vaccinated, we were
unable to participate in any of the handson animal work.