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Molecular Cytogenetics and Genomics Laboratory

Services - Genotyping

The Molecular Cytogenetics and Genomics Laboratory at Texas A&M University performs ready-to-run genotyping reactions.

For Genotyping

Client must prepare genotyping reactions as follows:
Add 10µl ABI HiDi formamide and 0.3µl of GeneScan LIZ size standardad to 0.5-1.0 µl of each genotyping reaction and vortex.

Plate Preparation (for genotyping):

  • For one injection (48 reactions) sample to be added in the 48 odd numbered wells (1, 3, 5, 7, 9, 11) in 3730 compatible 96 well plate.
  • For two injections (96 reactions) utilize all wells.

We run regularly scheduled validation checks of all our instruments with control samples to verify that all equipment is functioning properly. Therefore, if the run is successful for the other samples on the plate, and your sample fails, we don't accept responsibility for the failure. You will be charged for running both the failed samples and any reruns. We will be happy to help you troubleshoot your sequencing reactions. All samples are entered into a database that gives each entry an ID number for customer results and data storage purposes. So, a data sheet for each genotyping reaction should be filled up by the customer, which is available for genotyping (48 well, 96 well), and then sent by e-mail.

Turn-around Time

The turn-around time is 1-2 working days for ready-to-run samples. Occasional delays may occur if we experience unusually heavy loads or equipment failure.

Results

Results will be available as computer files. Results can be retrieved either directly from the facility or by e-mail. We can assist you in interpreting your results and trouble-shooting problems.

Data Storage

All data is backed up daily. Raw data is stored for 1 month. Analyzed data is permanently archived and backed up off-site.

Cost

Costs per injection (48 reaction /injection) are $96.00 (sequencing) and $72.00(genotyping) for ready-to run reactions.

Support:

Monday through Friday: 9 AM to 5 PM

Also available at the core:
BigDye Vesrsion 1.1 -80µl/tube@ $80.00
3730 Compatible 96 well Plate- 25per pack@$65.00
ABI HiDi Formamide-25ml bottle@$29.00
An account will be set up for each P.I. using the facility. Customers wishing to set up a new account should bring billing information.