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Molecular Cytogenetics and Genomics Laboratoy

Services - Sequencing

The Molecular Cytogenetics and Genomics Laboratory at Texas A&M University performs ready-to-run sequencing reactions. You do the sequencing reaction yourself and we run the samples on 3730 capillary sequencer.

Sample Submission

Bring us the re-suspended samples in 11µl of ABI HiDi formamide (in a tube wrapped with Aluminum foil) after column purification and drying. Alternatively, we can re-suspend your samples for at a flat rate of $10 per injection (48 reactions), up to 96 samples. You may send  your sample in sequencing plates, micro-centrifuge tubes or in PCR plates in dry condition, without exposing to direct light and at +40C, overnight.

Plate Preparation (for genotyping and sequencing):

  • For one injection (48 reactions) sample to be added in the 48 odd numbered wells (1, 3, 5, 7, 9, 11) in 3730 compatible 96 well plate.
  • For two injections (96 reactions) utilize all wells.

We run regularly scheduled validation checks of all our instruments with control samples to verify that all equipment is functioning properly. Therefore, if the run is successful for the other samples on the plate, and your sample fails, we don't accept responsibility for the failure. You will be charged for running both the failed samples and any reruns. We will be happy to help you troubleshoot your sequencing reactions. All samples are entered into a database that gives each entry an ID number for customer results and data storage purposes. So, a data sheet for each sequencing reaction should be filled up by the customer, which is available for sequencing (48 well, 96 well) and then sent by e-mail.

Turn-around Time

The turn-around time is 1-2 working days for ready-to-run samples. Occasional delays may occur if we experience unusually heavy loads or equipment failure.

Results

Results will be available as computer files. Results can be retrieved either directly from the facility or by e-mail.We can assist you in interpreting your results and trouble-shooting problems.

Data Storage

All data is backed up daily. Raw data is stored for 1 month. Analyzed data is permanently archived and backed up off-site.

Cost

Costs per injection (48 reaction /injection) are $96.00 (sequencing) and $72.00(genotyping) for ready-to run reactions.

Support:

Monday through Friday: 9 AM to 5 PM

Also available at the core:
BigDye Vesrsion 1.1 -80µl/tube@ $80.00
3730 Compatible 96 well Plate- 25per pack @ $65.00
ABI HiDi Formamide-25ml bottle@$29.00
An account will be set up for each P.I. using the facility. Customers wishing to set up a new account should bring billing information.