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Special Stains

The following is a list of special stains that the VIBS Histology Laboratory provides:

  1. Acid Fast Blue (AFB)
  2. Alcian Blue:-
    1. Alcian Blue for Mucosubstance pH 2.5
    2. Alcian Blue – Safranin O
  3. Mallory Aniline Blue Method for:-
    1. Collagen
    2. Astrocytes
  4. Congo Red
  5. Cresyl Violet Acetate
  6. Fite’s Stain
  7. Fontana-Masson
  8. Giemsa Stain:-
    1. May-Grunwald Method
    2. For Bone
    3. Modified
  9. Iron Stain:-
    1. Gomori’s Reaction
    2. Perl’s Method
    3. Prussian Blue
  10. Gram Stain – Brown and  Hoff’s modified Gram stain
  11. Gridley Stain
  12. Grimelius Stain
  13. Hematoxylin & Eosin (H&E) Stain
  14. Luxol Fast Blue (LFB)
  15. Melanin Bleach
  16. Mucicarmine Stain
  17. Oil Red O
  18. Periodic Acid Schiff’s (PAS):-
    1. With and without diastase
    2. Periodic Acid – Schiff – Orange G (TRIPAS) Stain
  19. Phosphotungstic Acid Hematoxylin (PTAH)
  20. Silver Stain:-
    1. Bielschowsky Technique for Axons and Neurofibrils
    2. Gomori Stain for Reticular Fibers
    3. Grocott Methenamine-Silver Nitrate Fungus Stain (GMS)
    4. Holmes’ Method
    5. Steiner’s Stain
    6. Periodic Acid Methenamine Silver Stain (PAM)
    7. Weil Method for Myelin
    8. Warthin Starry
  21. Toluidine Blue
    1. For Nerve cells
    2. For Mast cells
  22. Trichrome
    1. Gomori’s One Step Trichrome
    2. Masson’s Method
  23. Van Gieson’s stain
  24. Verhoeff’s elastic  stain
  25. Von Kossa

** Other special stains can be completed upon request. An extra charge may apply depending upon the reagents required. If there is a special stain needed that is not listed, please contact Lin Bustamante or Chaitali Mukherjee (979-845-3177) to discuss that particular special stain.