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Special Stains

The following is a list of special stains that the VIBS Histology Laboratory provides:

  1. Acid Fast Blue (AFB)
  2. Alcian Blue
    1. Alcian Blue for Mucosubstance pH 2.5
    2. Alcian Blue - Safranin O
  3. Aniline Blue
    1. Mallory Aniline Blue Method for Collagen Stain
    2. Mallory Aniline Blue Method for Astrocytes
  4. Congo Red
  5. Cresyl Violet Acetate
  6. Fettrot Method
    1. For Lipids
    2. For Neutral Fat
  7. Fite's Stain
  8. Fontana-Masson
  9. Giemsa Stain
    1. May-Grunwald Method
    2. For Bone
    3. Modified
  10. Iron Stain
    1. Gomori's Reaction
    2. Perl's Method
    3. Prussian Blue
  11. Gram Stain
    1. For Bacteria
    2. With Safranin
    3. Brown-Brenn
  12. Gridley Stain
  13. Grimelius Stain
  14. Hematoxylin & Eosin (H&E) Stain
    1. For Plastics
    2. For Mouse Eyes
    3. Gill's Hematoxylin
    4. Harris Hematoxylin
  15. Luxol Fast Blue (LFB)
  16. Melanin Bleach
  17. Methyl Green Pyrononin (MGP)
  18. Movat's Pentachrome
  19. Mucicarmine Stain
  20. Myleoperoxidase Stain
  21. Oil Red O
  22. Periodic Acid Schiff's (PAS)
    1. For Plastics
    2. With Diastase
    3. Periodic Acid - Schiff - Orange G (TRIPAS) Stain
  23. Phosphotungstic Acid Hematoxylin (PTAH)
  24. Richettsia Stain
    1. Ordway-Machiavello Method
    2. Pinkerton's Method
  25. Silver Stain
    1. Bielschowsky Technique for Axons and Neurofibrils
    2. Gomori Stain for Reticular Fibers
    3. Grocott Methenamine-Silver Nitrate Fungus Stain (GMS)
    4. Holmes' Method
    5. Steiner's Stain
    6. Periodic Acid Methenamine Silver Stain (PAM)
    7. Weil Method for Myelin
    8. Warthin Starry
  26. Toluidine Blue
    1. For Nerve cells
    2. For Mast cells
  27. Trichrome
    1. Mallory Method
    2. Gomori's One Step Trichrome
    3. Masson's Method
  28. Van Gieson's Stain
  29. Verhoeff's Elastic Stain
  30. Von Kossa

Other special stains can be completed upon request.  An extra charge may apply depending upon the reagents required for the special stains.