Standard Tissue Collection for Immersion Fixation
Prior to fixation, trim your tissue.
Fix the tissue.
- Most fixatives penetrate very slowly. No fixative will
penetrate more then 2-3 mm of solid tissue in 24 hours. Most
specimens should be no more than 4 mm for good fixation, and 3 mm
- If you are working with whole brains, large tumors, or some
large organ pieces, standard-sized tissue pieces may not be big
enough for your work. If you are not sure, we can help you decide
how large your tissue should be.
- If you must cut sections thicker than 4 mm, do not use
standard-sized cassettes. Standard-sized cassettes will squeeze
larger samples and cause under-processing. If you need larger
cassettes, come by our lab and we will supply you with them.
- Researchers are responsible for their choice of fixative and
fixation time. Our lab supplies at no extra cost 10%
Neutral-Buffered Formalin and Davidson's fixative.
- If tissues are submitted still in fixative, our lab will not be
responsible for fixation time.
- Be sure to follow the standard tissue submission
If you have any questions, contact us at:
Tel: (979) 845-3177 Fax: (979) 458-3499