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Scarlet Macaw Genome Project

Modern, next-generation gene sequencing technology makes it possible to sequence the DNA in the entire genome of any bird relatively quickly and cheaply.  Once the genome is completely sequenced and assembled it provides scientists with a guidebook to the biology of the species.  We chose to sequence the genome of a wild-caught female Scarlet macaw (Ara macao). We chose a wild-caught bird because its genes would reflect those in a wild population rather than a captive bred bird in which we had no assurance that its parents were unrelated.  We chose a female because in birds, that is the sex with two different sex chromosomes (Z and W), and we could therefore determine the gene sequences in both of them.  We chose a Scarlet macaw since that is a species we have been studying in the wild for many years at the Tambopata Research Center and know a lot about its biology.

We sequenced a single bird, “Neblina” who lives at Blank Park Zoo in Des Moines, Iowa. She probably originated in Brazil and was seized from bird smugglers by USFWS around 1995.  The results of the study were published in the journal PLoS one in May 2013. Volume 8, Issue 5, e62415, in a paper entitled “A multi-platform draft de novo genome assembly and comparative analysis for the Scarlet macaw (Ara macao)”.

DNA molecules consist of long chains of molecules called nucleotides or bases. The sequencing of her DNA revealed that Neblina’s genome contained slightly over a billion bases (1.035 gigabases to be precise).  Using on-line databases we identified over 14,000 genes. There will be many more when her genome is completely annotated.   Its called a draft genome since this is still a work in progress.  As expected, her genome contained many unique genes that probably control brain development, speech, intelligence and longevity.

The complete Scarlet macaw draft genome is freely available here on-line for anyone who would like to investigate further.  We encourage other scientists to examine the genome in detail and derive as much useful information from it as possible.

Sincerely,
Christopher M. Seabury
Ian R. Tizard

 

Blue folder iconSimple De Novo Assembly (302.11 MB)

'Read Me' file for Simple De Novo

 

Blue folder iconScaffolded De Novo Assembly (279.23 MB)

'Read Me' files for scaffolded De Novo:  File1 , File 2

 

Blue folder iconAnnotation (240.54 MB)

 

Blue folder iconSupplemental Data