Skip Navigation

Digital Imaging

The Department of Veterinary Pathobiology has acquired and assembled a fully functional core facility for computer-based acquisition of images, their editing and analysis, as well as other shared research instrumentation.

These core assets are managed by:

roths

John B. Roths
Assoc. Research Scientist
322 VMR Building
jroths@cvm.tamu.edu

979-862-1871

 

He will provide interested Department faculty, staff and students with instruction on using the hardware and software and assist in experimental design, as needed.  Training is required, as indicated below, for some instrumentation prior to independent use.

Microscopy and Digital Imaging Components


VMA/VMS Buildings

Olympus IX70 System (214D VMA)

This system is equipped with superb optics including 10X, 20X and 40X "Dry" objectives as well as 20X, 60X and 100X "Oil" objectives.  The latter are all apochromatic, including the 1.4 N.A. 60X, used for high resolution.  Two condenser systems are available, allowing for lower magnification tissue culture imaging as well as high magnification tissue/cellular imaging.  The IX70 is used for transmitted brightfield and reflected light (epi-) fluorescence imaging, with filter sets appropriate for DAPI, FITC, TX-RED and Calcifluor. Polarization and Nomarski Differential Interference Contrast (D.I.C.) Micrsocopy can also be performed.

Please contact J. Roths for scheduling a training session.

Olympus IX70 System

Olympus VANOX AH2 microscope (204 VMA)

This Vanox It is solely equipped for brightfield microscopy using a Diagnostic Imaging Spot Insight camera.

Olympus VANOX AH2 Microscope

Alpha Imager 2200 "Gel Doc" System (214D VMA):

Primarily used for documentation of ethidium bromide gels. This older imaging unit is considered a "back up". Please use the newer Fluorchem System in 212B VMS if possible. A separate transilluminator is provided in 214D for gel "cutting" (band isolation).

Alpha Imager 220 "Gel Doc" System

Alpha-Innotech Fluorchem System (212B VMA)

This system has UV transillumination, white light transillumination and reflected white light illumination for a variety of imaging needs.  When using UV transillumination, filters for EtBr, SYBR Green, and Hoechst Blue are selectable. The Fluorchem uses a low-light, highly sensitive 16-bit camera for image acquisition using a Windows PC.  A Sony UDP800 Thermal printer produces output useful for lab notebooks. The camera lens has an extensive range of focal lengths permitting imaging small PCR gels up to full sized Southern blots (23x25cm). A very large aperture (F-Stop 1.2) is ideal for weak fluorescent signals.  A training session is required before issuance of a "user account" and password.

Alpha-Innotech Fluorchem System

Reflected and Transmitted Light Flatbed Scanners

VTPB has several flatbed scanners available in 204 VMA, 214D VMA and 210 VMR that are suitable for scientific scanning, having been density-calibrated using Kodak calibrated step-tablets, for both reflected and transmitted light.

The Epson Scanner in 214D VMA has been modified for scanning the entirety of  optically clear, flat-bottom microplates. It also works well for the scanning of electron microscopy negatives as well as scanning Coomassie blue polyacrylamide gels, for example.

Reflected and Transmitted Light Flatbed Scanners

VMD Building

Olympus VS120 Virtual Microscopy System. (54X VMD)

The VS120 is designed to acquire, manually or automatically, up to five full 1"x3" microslide images of tissues of user-determined magnification. With the available OlyVia (PC only) software, one can visualize these tissue sections in a computer-based virtualized microscopy setting.  A network-based database and Web Browser "front end" is being planned, thus enabling distance learning.

Olympus VS120 Virtual Microscopy System

Optronics Microcast HD Studio (50X VMD)

The Microcast has the unique ability to collect High Definition (HD) 1080p images or videos from the Olympus BX51 brightfield microscope for projection or collection to a USB-connected flash drive. Such widefield images can be ideal for publication or the acquired video can be used for teaching histology, cytology and pathology.

Optronics Microcast HD Studio

VMR Building

Olympus IX71 System (210 VMR)

This system is equipped with achromatic optics including 2X, 10X , 20X and 40X "Dry" objectives. This system is principally used for brightfield and fluorescence imaging of cultured cells in microplates.  The IX71 includes filter sets appropriate for DAPI, FITC, and TX-RED. Polarization and Nomarski Differential Interference Contrast (D.I.C.) Microscopy can also be performed.

Please contact J. Roths for scheduling a training session.

Olympus IX71 System

Olympus VANOX AH3 microscope (210 VMR)

This is a superb upright microscope for high-resolution brightfield, epi-fluorescence, polarization and Nomarski D.I.C. microscopy. The quality of the D.I.C. images is the best of the systems in our department.  As usual, the fluorescence filter sets include: DAPI, FITC, and TX-RED. Please contact J. Roths for scheduling a training session. See below picture (right).

Olympus VANOX AH3 Microscope

Olympus Research Stereo SZH microscope (210 VMR)

This stereo zoom microscope is fitted with PlanAPO lenses for superb low power imaging. It is fit with a Spot Insight Color Camera and has transmitted as well as fiber-optic and ring-light incident illumination. See above picture (left).

Diagnostic Imaging, Inc. "Spot RT" cooled CCD, filter selectable ("slider) high resolution cameras such as the Spot RT3 Slider are in use on the Vanox, IX70 and IX71 microscopes.

The SPOT RT3 Slider enables both true color imaging and high sensitivity monochrome imaging in a single camera. Electronic circuitry refinements and deep cooling provide clean, uniform backgrounds when light is limited. The flexible RT3 Slider is ideal for brightfield color imaging, fluorescence microscopy, FISH, GFP imaging, and immunofluorescence.

Spot v5.1 Advanced imaging software is available on either Macintosh or Windows computers used with the Vanox, IX70 or IX71 systems.

Olympus Research Sterep SZH Microscope

CompuCyte Laser Scanning Cytometer with Olympus BX60 microscope (210 VMR)

The LSC uses laser excitation and scanning and PMT signal detection to measure properties of cells based on fluorescence signals directly from samples on microscope slides.  Acquisition and analysis is managed by WinCyte software on a Windows PC. Data can be exported as FCS format files, similar to that of Flow Cytometers.

CompuCyte Laser Scanning Cytometer with Olympus BX60 Microscope

Fuji Medical Systems BAS-1800-II Bio-Imager (210 VMR)

Designed for radioisotopic detection of H3, C14, P32, P33, S35, e.g., on Fujifilm Imaging Plates using cassette exposure. Used for 1D Gel Electrophoresis, 2D Gel Electrophoresis, RFLP, Differential Display, Detection of PCR Products, High-Density Array, In situ Hybridization, Histology, Dot/Slot Blots, Southern- Northern- Western Blots, CAT Assay, Whole Body Autoradiography, TLC.

Fuji Medical Systems BAS-1800-II Bio-Imager

Bio-Rad 1024 Confocal and Multiphoton Microscopy (325 VMR)

These technologies are available as a joint effort of Drs. Schroeder (VTPP) and Kier (VTPB) and their respective departments.  Based on a Bio-Rad 1024 Krypton-Argon laser Confocal Microscope, this system has been extended for Multi-Photon Microscopy using an Argon-Ion pumped, mode-locked, tunable Ti:Sapphire far red/infrared laser (650 - 1100nm).  The Zeiss Axiophot 135 Inverted Microscope is fit with an epi-fluorescence illuminator and a completely self-contained cell culture incubation system (temperature, C02, humidity controlled) for extended length microscopy and image acquisition sessions.  Image acquisition and analysis employs Bio-Rad "LaserSharp" software.  Independent use of this system requires participation in VTPP-677 (Spring semester) and mentoring.

Contact: A. McIntosh (862-1434) or J. Roths (862-1871).

Bio-Rad 1024 Confocal and Multiphoton Microscopy

Alpha-Innotech Fluorchem System (210 VMR)

This system has UV transillumination, white light transillumination and reflected white light illumination for a variety of imaging needs.  When using UV transillumination, filters for Ethidium Bromide, SYBR Green, GPF, and Hoechst Blue are selectable. The Fluorchem uses a low-light, high-sensitivity 8 to 16-bit camera for image acquisition using a Windows PC. This unit is capable of measuring chemiluminescence and imaging of GFP. A Sony UDP800 Thermal printer produces output useful for lab notebooks. An training session is required before issuance of a "user account" and password.Alpha-Innotech Fluorchem System

A variety of other imaging instrumentation is available in 210 VMR including a Mitsubishi Flatbed scanner for both incident and transmitted scanning. This scanner has been density calibrated for use in quantitation of gel images.  In addition (see picture below), we have the capability of acquiring images from 35 mm slides, via magazine loading on the PowerSlide 3600 scanner.  In addition, the Nikon Coolscan 5000 is equipped with an adapter for scanning 1"x3" microscope slide images. This provides for extremely high quality, flat, evenly illuminated "low mag" images of sectioned tissue. In addition we have a 1280 x 1024 dpi hand-held Scalar ProScope Microscope with 1 to 100X lenses. The LED illumination and surface-focused design is ideal for imaging skin or other surfaces.

Coolscan, Etc

 

Other Shared Scientific Instrumentation


VMA/VMS Building

Molecular Devices Spectra Max Gemini EM Fluorescence Microplate Reader (212B VMS)

A flexible tool for fluorescence assays reading 6 to 384-well microplates.  This top and bottom reader uses dual monochromators for variable wavelength selection between 250 nm and 850 nm thus eliminating the need for searching out the right pair of excitation and emission filters.  Incubation temperatures are adjustable from ambient to 47 ºC.  The Gemini EM uses SoftMax Pro software for microplate data acquisition and analysis on a Windows PC.

Molecular Devices Spectra Max Gemini EM

VMR Building

Beckman Coulter LS 6500 Scintillation counter (115 VMR)

The LS 6500 is a bench top liquid scintillation counter. It features a digital signal processor and a 32,768 multichannel analyzer that provides a high effective resolution. Ideal for analyzing the reactivity level in radioactively-tagged samples. It has a resolution of 0.06 keV per channel. Designed for: Beta emission: C-14, H-3 (tritium), P-32, P-33, S-35; Gamma emission: (auger electrons) Cr-51, Cs-137 and I-125.

Beckman Coulter LS 6500 Scintillation counter

Imaging Analysis Software Support

John Roths will be glad to assist in tutoring faculty, staff and students in using all the installed hardware-specific image and data acquisition and analysis software. For post image-acquisition, assistance in image processing and densitometric and morphometric analysis is available using both commercial (MetaMorph, Spot Advanced and CoLocalizer Pro) and Open Source software (such as ImageJ, FIJI, Cell Profiler, ImageSXM, JMicroVision, Micro-Manager, OMERO, OsiriX, and others). Please contact J. Roths for assistance with experimental design, protocol development, image processing and analysis and data analysis.