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Flow Cytometry

The Flow Cytometry lab facility is located in room 257, Veterinary Medical Research (VMR) Building Annex.

The facility operator is Dr. Roger Smith III.
For more information, or to arrange assays, contact Dr. Smith: email or 1-979-845-5167.

Becton Dickinson FACSCalibur

Argon (488nm) Laser
3-color fluorescence

Beckman Coulter MoFlo Astrios Cell Sorter

Violet (405nm), Blue (488nm), & Red (642nm) Lasers
11-color fluorescence
Cell Sorting @ up to 70K cells per second

FACSCalibur MoFlo Astrios

Assays Supported

  • Immunofluorescence (surface & intracellular antigens, quantitative)
  • Reporter Gene Expression
  • DNA & Cell Cycle Analysis (using ModFit LT software)
  • Cell Viability
  • Apoptosis (annexin V binding, caspase activation, TUNEL)
  • Cell Proliferation (CFSE)
  • Phagocytosis of fluorescent beads
  • Cell Sorting on any fluorescence measurements