Flow Cytometry
The facility operator is Dr. Roger Smith
III (rosmith@cvm.tamu.edu),
1-979-845-5167. For more information, or to arrange assays, contact
Dr. Smith.
Location
The Flow Cytometry facility is located in room 257, Veterinary
Medical Research (VMR) Building Annex.
Instrumentation
- Becton-Dickinson FACSCalibur™
- argon laser (488nm)
- 3-color fluorescence
- Becton-Dickinson FACSVantage SE™(not yet transferred to the new
lab)
- Lasers:
- argon (488nm)
- HeNe (633nm)
- solid-state UV (355nm)
- up to 6-color fluorescence
- with TurboSort option
FACSCalibur
FACSVantage
Software
- CellQuest™ (Becton-Dickinson)
- FlowJo™ (TreeStar)
- ModFit LT™ (Verity Software House)
Capabilities
Detection of the following fluorochromes:
- Both FACSCalibur & FACSVantage SE:
- Fluorescein, CFSE, Alexa Fluor 488
- GFP
- R-phycoerythrin
- R-phycoerythrin-cyanine 5 (PE-Cy5) tandem dye
- Peridinin chlorophyll protein (PerCP)
- Propidium iodide
- 7-aminoactinomycin D
- Doxorubicin (adriamycin)
- Rhodamine 123
- and others (please check)
- FACSVantage SE only:
- Allophycocyanin (APC)
- Cyanine 5
- Alexa Fluor 647
- Alexa Fluor 700
- future: Hoechst dyes (33528, 33342)
- future: 4',6-diamidino-2-phenylindole (DAPI)
- future: Alexa Fluor 350
- and others (please check)
- Cell Sorting
Assays Supported
- Immunofluorescence (surface and intracellular antigens,
quantitative)
- DNA and cell cycle content analysis (using ModFit LT
software)
- Apoptosis (annexin V binding, caspase activation, TUNEL)
- Cell Proliferation (CFSE)
- Phagocytosis of fluorescent beads
- Functional expression of p-glycoprotein (using
doxorubicin)
User Fees
- Data Acquisition: $50 per hour ($15 minimum fee)
- Cell Sorting: $75 per hour (plus $100 set up fee)