Flow Cytometry
The Flow Cytometry lab facility is located in room 257,
Veterinary Medical Research (VMR) Building Annex.
The facility operator is Dr. Roger Smith
III.
For more information, or to arrange assays, contact Dr. Smith: email or 1-979-845-5167.
Becton Dickinson FACSCalibur
Argon (488nm) Laser
3-color fluorescence
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Beckman Coulter MoFlo Astrios Cell Sorter
Violet (405nm), Blue (488nm), & Red (642nm) Lasers
11-color fluorescence
Cell Sorting @ up to 70K cells per second
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Assays Supported
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- Immunofluorescence (surface &
intracellular antigens, quantitative)
- Reporter Gene Expression
- DNA & Cell Cycle Analysis
(using ModFit LT software)
- Cell Viability
- Apoptosis (annexin V binding,
caspase activation, TUNEL)
- Cell Proliferation (CFSE)
- Phagocytosis of fluorescent
beads
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- Cell Sorting on any fluorescence
measurements
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