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Blanca Lupiani

Professor and Associate Dean of Faculties

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Phone: (979) 458-0591

Mailstop: 4467

Department: VTPB

Photo of Lupiani, Blanca


  • Ph.D. Molecular Virology, University of Maryland 1994
  • B.S. Molecular Biology, University Santiago de Compostela, Spain 1988

Scholarly Interests

Research in my laboratory focuses on better understanding the ecology and evolution of avian influenza and avian paramyxoviruses. By carrying out surveillance and characterization of viruses isolated from wild bird populations from the wintering grounds of the Central Flyway (Texas Coast), we are able to better understand how these viruses are maintained in nature and how they evolve to adapt to new hosts. Molecular characterization of the viruses, together with ecological data are used for epidemiological and modeling studies. Another aspect of my research includes the study of molecular mechanisms of pathogenesis of avian influenza viruses. Using reverse genetics, specific mutations are introduced in the avian influenza virus genome in order to study their role in virus pathogenesis as well as to determine gene function. Using genomic approaches the host/virus interaction is also being studied. We are also interested in understanding molecular mechanisms of pathogenesis of Mareks disease virus, a chicken oncogenic alphaherpesvirus, and in the development of improved vaccines to control this important poultry pathogen.


Cloning of a very virulent plus, 686 strain of Marek's disease virus as a bacterial artificial chromosome.
Reddy SM, Sun A, Khan OA, Lee LF, Lupiani B
Avian Dis. 57(2 Suppl):469-73.
A Marek's disease virus vIL-8 deletion mutant has attenuated virulence and confers protection against challenge with a very virulent plus strain.
Cui X, Lee LF, Hunt HD, Reed WM, Lupiani B, Reddy SM
Avian Dis. 49(2):199-206.
Characterization of a very virulent Marek's disease virus mutant expressing the pp38 protein from the serotype 1 vaccine strain CVI988/Rispens.
Lee LF, Cui X, Cui Z, Gimeno I, Lupiani B, Reddy SM
Virus Genes. 31(1):73-80.
Expansion of a unique region in the Marek's disease virus genome occurs concomitantly with attenuation but is not sufficient to cause attenuation.
Silva RF, Reddy SM, Lupiani B
J Virol. 78(2):733-40.
Pathogenicity of two recombinant avian leukosis viruses.
Lupiani B, Williams SM, Silva RF, Hunt HD, Fadly AM
Avian Dis. 47(2):425-32.
Diagnosis of myeloid leukosis induced by a recombinant avian leukosis virus in commercial white leghorn egg laying flocks.
Gingerich E, Porter RE, Lupiani B, Fadly AM
Avian Dis. 46(3):745-8.
Rescue of a pathogenic Marek's disease virus with overlapping cosmid DNAs: use of a pp38 mutant to validate the technology for the study of gene function.
Reddy SM, Lupiani B, Gimeno IM, Silva RF, Lee LF, Witter RL
Proc Natl Acad Sci U S A. 99(10):7054-9.
Marek's disease virus down-regulates surface expression of MHC (B Complex) Class I (BF) glycoproteins during active but not latent infection of chicken cells.
Hunt HD, Lupiani B, Miller MM, Gimeno I, Lee LF, Parcells MS
Virology. 282(1):198-205.
Protein-coding content of the sequence of Marek's disease virus serotype 1.
Lupiani B, Lee LF, Reddy SM
Curr Top Microbiol Immunol. 255:159-90.
Identification and characterization of recombinant subgroup J avian leukosis viruses (ALV) expressing subgroup A ALV envelope.
Lupiani B, Hunt H, Silva R, Fadly A
Virology. 276(1):37-43.
Further characterization of the gonad-specific virus of corn earworm, Helicoverpa zea.
Raina AK, Adams JR, Lupiani B, Lynn DE, Kim W, Burand JP, Dougherty EM
J Invertebr Pathol. 76(1):6-12.
Development and use of a PCR assay for detection of the reproductive virus in wild populations of helicoverpa zea (Lepidoptera: noctuidae)
Lupiani B, Raina AK, Huber C
J Invertebr Pathol. 73(1):107-12.
Cloning, sequence analysis and expression of the major outer capsid protein gene of an aquareovirus.
Lupiani B, Reddy SM, Subramanian K, Samal SK
J Gen Virol. 78 ( Pt 6):1379-83.
Sequence analysis of genome segment 10 encoding the major outer capsid protein (VP7) of genogroup B aquareovirus and its relationship with the VP7 protein of genogroup A aquareovirus.
Lupiani B, Reddy SM, Samal SK
Arch Virol. 142(12):2547-52.
Mapping the domains on the phosphoprotein of bovine respiratory syncytial virus required for N-P interaction using a two-hybrid system.
Mallipeddi SK, Lupiani B, Samal SK
J Gen Virol. 77 ( Pt 5):1019-23.
Characterization of 2 aquareovirus proteins.
Samal SK, Subramanian K, Lupiani B
Vet Res. 26(5-6):388-93.
Genetic analysis of aquareoviruses using RNA-RNA blot hybridization.
Lupiani B, Hetrick FM, Samal SK
Virology. 197(1):475-9.
Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe.
Subramanian K, Lupiani B, Hetrick FM, Samal SK
J Clin Microbiol. 31(6):1612-4.
Biochemical and serological characterization of Carnobacterium spp. isolated from farmed and natural populations of striped bass and catfish.
Baya AM, Toranzo AE, Lupiani B, Li T, Roberson BS, Hetrick FM
Appl Environ Microbiol. 57(11):3114-20.
Heterogeneity in the genome RNAs and polypeptides of five members of a novel group of rotavirus-like viruses isolated from aquatic animals.
Samal SK, Dopazo CP, Subramanian K, Lupiani B, Mohanty SB, Hetrick FM
J Gen Virol. 72 ( Pt 1):181-4.
Fish viral infections in northwest of Spain.
Ledo A, Lupiani B, Dopazo CP, Toranzo AE, Barja JL
Microbiologia. 6(1):21-9.
RNA-seq analysis revealed novel genes and signaling pathway associated with disease resistance to avian influenza virus infection in chickens.
Wang Y, Lupiani B, Reddy SM, Lamont SJ, Zhou H
Poult Sci. 93(2):485-93.
Cross-sectional serosurvey of avian influenza antibodies presence in domestic ducks of Kathmandu, Nepal.
Karki S, Lupiani B, Budke CM, Manandhar S, Ivanek R
Zoonoses Public Health. 61(6):442-8.
H7N9 Influenza A virus in Turkeys in Minnesota.
Lebarbenchon C, Pedersen JC, Sreevatsan S, Ramey AM, Dugan VG, Halpin RA, Ferro PJ, Lupiani B, Enomoto S, Poulson RL, Smeltzer M, Cardona CJ, Tompkins SM, Wentworth DE, Stallknecht DE, Brown JD
J Gen Virol.
Deletion of Marek's disease virus large subunit of ribonucleotide reductase impairs virus growth in vitro and in vivo.
Sun A, Lee LF, Khan OA, Heidari M, Zhang H, Lupiani B, Reddy SM
Avian Dis. 57(2 Suppl):464-8.
Insertion of reticuloendotheliosis virus long terminal repeat into the genome of CVI988 strain of Marek's disease virus results in enhanced growth and protection.
Lupiani B, Lee LF, Kreager KS, Witter RL, Reddy SM
Avian Dis. 57(2 Suppl):427-31.
Avian influenza virus surveillance in hunter-harvested waterfowl, Texas coast, September 2009-January 2010.
Ferro PJ, Khan O, Peterson MJ, Batchuluun D, Reddy SM, Lupiani B
Avian Dis. 56(4 Suppl):1006-9.
Avian influenza virus investigation in wild bobwhite quail from Texas.
Ferro PJ, Khan O, Vuong C, Reddy SM, LaCoste L, Rollins D, Lupiani B
Avian Dis. 56(4 Suppl):858-60.
Role of nonmigratory mottled ducks (Anas fulvigula) as sentinels for avian influenza surveillance.
Rollo SN, Ferro PJ, Peterson MJ, Ward MP, Ballard BM, Lupiani B
J Zoo Wildl Med. 43(1):168-70.
Detection and characterization of a distinct bornavirus lineage from healthy Canada geese (Branta canadensis).
Payne S, Covaleda L, Jianhua G, Swafford S, Baroch J, Ferro PJ, Lupiani B, Heatley J, Tizard I
J Virol. 85(22):12053-6.
Simultaneous detection of avian influenza virus NP and H5 antibodies in chicken sera using a fluorescence microsphere immunoassay.
Lupiani B, Mozisek B, Mason PW, Lamichhane C, Reddy SM
Avian Dis. 54(1 Suppl):668-72.
Comparison of real-time reverse transcription-PCR and virus isolation for estimating prevalence of avian influenza virus in hunter-harvested wild birds at waterfowl wintering grounds along the Texas mid-Gulf Coast (2005-2006 through 2008-2009).
Ferro PJ, Peterson MJ, Merendino T, Nelson M, Lupiani B
Avian Dis. 54(1 Suppl):655-9.
Both homo and heterodimers of Marek's disease virus encoded Meq protein contribute to transformation of lymphocytes in chickens.
Suchodolski PF, Izumiya Y, Lupiani B, Ajithdoss DK, Lee LF, Kung HJ, Reddy SM
Virology. 399(2):312-21.
Avian influenza viral nucleocapsid and hemagglutinin proteins induce chicken CD8+ memory T lymphocytes.
Singh S, Briles WE, Lupiani B, Collisson EW
Virology. 399(2):231-8.
Evaluation of a 384-well format for high-throughput real-time reverse transcription polymerase chain reaction for avian influenza testing.
Ferro PJ, Osterstock J, Norby B, Fosgate GT, Lupiani B
J Vet Diagn Invest. 21(5):679-83.
Preliminary study for evaluation of avian influenza virus inactivation in contaminated poultry products using electron beam irradiation.
Brahmakshatriya V, Lupiani B, Brinlee JL, Cepeda M, Pillai SD, Reddy SM
Avian Pathol. 38(3):245-50.
A multiplexed immunoassay for detection of antibodies against avian influenza virus.
Watson DS, Reddy SM, Brahmakshatriya V, Lupiani B
J Immunol Methods. 340(2):123-31.
Homodimerization of Marek's disease virus-encoded Meq protein is not sufficient for transformation of lymphocytes in chickens.
Suchodolski PF, Izumiya Y, Lupiani B, Ajithdoss DK, Gilad O, Lee LF, Kung HJ, Reddy SM
J Virol. 83(2):859-69.
The history of avian influenza.
Lupiani B, Reddy SM
Comp Immunol Microbiol Infect Dis. 32(4):311-23.
Avian influenza surveillance in hunter-harvested waterfowl from the Gulf Coast of Texas (November 2005-January 2006).
Ferro PJ, El-Attrache J, Fang X, Rollo SN, Jester A, Merendino T, Peterson MJ, Lupiani B
J Wildl Dis. 44(2):434-9.
Molecular and biological characterization of a naturally occurring recombinant subgroup B avian leukosis virus with a subgroup J-like long terminal repeat.
Lupiani B, Pandiri AR, Mays J, Hunt HD, Fadly AM
Avian Dis. 50(4):572-8.
Marek's disease virus-encoded Meq gene is involved in transformation of lymphocytes but is dispensable for replication.
Lupiani B, Lee LF, Cui X, Gimeno I, Anderson A, Morgan RW, Silva RF, Witter RL, Kung HJ, Reddy SM
Proc Natl Acad Sci U S A. 101(32):11815-20.
Identification and in vitro characterization of a Marek's disease virus-encoded Ribonucleotide reductase.
Lee LF, Heidari M, Sun A, Zhang H, Lupiani B, Reddy S
Avian Dis. 57(2):178-87.
Properties of a meq-deleted rmd5 Marek's disease vaccine: protection against virulent MDV challenge and induction of lymphoid organ atrophy are simultaneously attenuated by serial passage in vitro.
Lee LF, Kreager K, Heidari M, Zhang H, Lupiani B, Reddy SM, Fadly A
Avian Dis. 57(2 Suppl):491-7.
Associations of chicken Mx1 polymorphism with antiviral responses in avian influenza virus infected embryos and broilers.
Wang Y, Brahmakshatriya V, Lupiani B, Reddy S, Okimoto R, Li X, Chiang H, Zhou H
Poult Sci. 91(12):3019-24.
Integrated analysis of microRNA expression and mRNA transcriptome in lungs of avian influenza virus infected broilers.
Wang Y, Brahmakshatriya V, Lupiani B, Reddy SM, Soibam B, Benham AL, Gunaratne P, Liu HC, Trakooljul N, Ing N, Okimoto R, Zhou H
BMC Genomics. 13:278.
Cell culture attenuation eliminates rMd5ΔMeq-induced bursal and thymic atrophy and renders the mutant virus as an effective and safe vaccine against Marek's disease.
Lee LF, Heidari M, Zhang H, Lupiani B, Reddy SM, Fadly A
Vaccine. 30(34):5151-8.
A duplex real-time polymerase chain reaction assay for the simultaneous detection of long terminal repeat regions and envelope protein gene sequences of Reticuloendotheliosis virus in avian blood samples.
Sun F, Ferro PJ, Lupiani B, Kahl J, Morrow ME, Flanagan JP, Estevez C, Clavijo A
J Vet Diagn Invest. 23(5):937-41.
Development of DIVA (differentiation of infected from vaccinated animals) vaccines utilizing heterologous NA and NS1 protein strategies for the control of triple reassortant H3N2 influenza in turkeys.
Wang L, Qin Z, Pantin-Jackwood M, Faulkner O, Suarez DL, Garcia M, Lupiani B, Reddy SM, Saif YM, Lee CW
Vaccine. 29(45):7966-74.
Evaluation of factors affecting vaccine efficacy of recombinant Marek's disease virus lacking the Meq oncogene in chickens.
Lee LF, Zhang H, Heidari M, Lupiani B, Reddy SM
Avian Dis. 55(2):172-9.
Ingestion of lead and nontoxic shot by Green-winged Teal (Anas crecca) and Northern Shovelers (Anas clypeata) from the mid-Gulf Coast of Texas, USA.
Garrison DA, Fedynich AM, Smith AJ, Ferro PJ, Butler DA, Peterson MJ, Lupiani B
J Wildl Dis. 47(3):784-6.
Effects of bacterial microflora of the lower digestive tract of free-range waterfowl on influenza virus activation.
King MD, Guentzel MN, Arulanandam BP, Bodour AA, Brahmakshatriya V, Lupiani B, Chambers JP
Appl Environ Microbiol. 77(12):4119-25.
Multiyear surveillance for avian influenza virus in waterfowl from wintering grounds, Texas coast, USA.
Ferro PJ, Budke CM, Peterson MJ, Cox D, Roltsch E, Merendino T, Nelson M, Lupiani B
Emerg Infect Dis. 16(8):1224-30.
Characterization and evaluation of avian influenza NS1 mutant virus as a potential live and killed DIVA (differentiating between infected and vaccinated animals) vaccine for chickens.
Brahmakshatriya VR, Lupiani B, Reddy SM
Vaccine. 28(12):2388-96.
Comparative evaluation of vaccine efficacy of recombinant Marek's disease virus vaccine lacking Meq oncogene in commercial chickens.
Lee LF, Kreager KS, Arango J, Paraguassu A, Beckman B, Zhang H, Fadly A, Lupiani B, Reddy SM
Vaccine. 28(5):1294-9.
Identification of differentially expressed miRNAs in chicken lung and trachea with avian influenza virus infection by a deep sequencing approach.
Wang Y, Brahmakshatriya V, Zhu H, Lupiani B, Reddy SM, Yoon BJ, Gunaratne PH, Kim JH, Chen R, Wang J, Zhou H
BMC Genomics. 10:512.
Proteolytic bacteria in the lower digestive tract of poultry may affect avian influenza virus pathogenicity.
King MD, Guentzel MN, Arulanandam BP, Lupiani B, Chambers JP
Poult Sci. 88(7):1388-93.
In vitro characterization of the Meq proteins of Marek's disease virus vaccine strain CVI988.
Ajithdoss DK, Reddy SM, Suchodolski PF, Lee LF, Kung HJ, Lupiani B
Virus Res. 142(1-2):57-67.
Recombinant Marek's disease virus (MDV) lacking the Meq oncogene confers protection against challenge with a very virulent plus strain of MDV.
Lee LF, Lupiani B, Silva RF, Kung HJ, Reddy SM
Vaccine. 26(15):1887-92.
Acquisition, persistence, and species susceptibility of the Hz-2V virus.
Raina AK, Lupiani B
J Invertebr Pathol. 93(2):71-4.

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