Katrin Hinrichs DVM, PhDKatrin Hinrichs DVM, PhD

Professor and Patsy Link Chair in Mare Reproductive Studies

Dr. Katrin Hinrichs graduated from the University of California, Davis School of Veterinary Medicine in 1978. After private practice, she spent six years at the University of Pennsylvania’s New Bolton Center completing a residency and PhD. There, she began her research on hormonal requirements for pregnancy and embryo transfer in the mare. She spent the next 10 years at Tufts University School of Veterinary Medicine, teaching and conducting research on oocyte maturation and fertilization in the horse. Hinrichs joined the faculty at the Texas A&M College of Veterinary Medicine & Biomedical Sciences in 1998; she has a joint appointment in Veterinary Physiology and Pharmacology and Large Animal Clinical Sciences, and was named the Patsy Link Chair in Mare Reproductive Studies in 2005.

Dr. Hinrichs is best known to the horse-owning world for her unique contributions to equine cloning and in vitro fertilization (intracytoplasmic sperm injection, or ICSI). She and her group cloned the first horse in North America, and the third in the world. Their continued efforts have resulted in one of the highest reported success rates for production of live offspring following nuclear transfer in any species. Her laboratory has also pioneered many techniques to improve the success and clinical application of ICSI. In addition, Dr. Hinrichs has contributed to the understanding of many other applicable procedures in equine assisted reproduction: helping to define requirements for equine embryo development; establishment of improved techniques to maintain viability of oocytes from injured or dying horses, so they may be transported to a specialty referral center for fertilization; improving understanding of the variables affecting oocyte maturation; development of a technique to biopsy horse embryos to determine the presence or absence of genetically-related diseases while maintaining the viability of the developing embryo; and establishment of techniques for improving success of embryo cryopreservation in horses. Her contributions have been widely employed by equine laboratories throughout the world.

Kindra RaderKindra Rader

Program Coordinator

Ms. Kindra Rader is originally from Cheyenne, Wyoming. She attended Laramie County Community College, where she competed on the horse show and horse judging teams while obtaining her Associates Degree in both Equine Science and Equine Training Management. In 2012, she obtained her Bachelors of Science degree in Animal Science at Oklahoma State University. Ms. Rader breeds quality reining horse prospects and has worked in numerous positions within the equine industry, including yearling sales preparation for Vista Equine Colorado, Royal Vista Ranches, and Lazy E Ranch with attendance to select yearling sales across the country. She has also served as foaling attendant for Royal Vista Ranches LLC, and breeding farm manager for Fox Meadow Farm Reining Horses in Oklahoma. This has helped her build a network of friends and mentors that have fueled her passion for horses and expanded her knowledge of horsemanship, equine physiology and the horse industry. Ms. Rader began working as a Technician at the Equine Embryo Laboratory at Texas A&M University in 2013 and now serves as the Program Coordinator for the Laboratory. Ms. Rader oversees all aspects of the clinical ICSI program including but not limited to transvaginal follicle aspiration procedures, oocyte recovery from isolated ovaries post mortem, media preparation, oocyte maturation, and coordination of embryo transfer, vitrification, and genetic biopsy procedures. Ms. Rader also serves as the laboratory liaison between the Equine Embryo Laboratory, the Large Animal Hospital, and the Section of Theriogenology.

Dr. Joao Luna Joao Luna, DVM

Research Assistant

Dr. Joao Brom-de-Luna obtained his DVM in Rio de Janeiro, Brazil in 2013. He gained veterinary experience in equine reproductive clinics and assisted reproduction in Brazil before joining Texas A&M University as a Research Scholar in 2014. Dr. Luna became a Research Assistant at the Equine Embryo Laboratory in 2015, developing methods to use flow-cytometric cell sorting to isolate somatic cells from from equine frozen semen for use in cloning (Brom-de-Luna et al., 2018a), and gaining experience in transvaginal follicle aspiration, oocyte manipulation and maturation, and ICSI. His advances in ICSI and embryo culture techniques have allowed him to obtain among the highest blastocyst production rates yet reported for equine ICSI (e.g. 40%, Brom-de-Luna et al., 2018b; 48%, Salgado et al., 2018; 54%, Brom-de-Luna et al., 2019). Because of his expertise in ICSI, Dr. Luna was invited to write the chapter on oocyte maturation, ICSI and embryo development for the upcoming book, Equine Assisted Reproduction. Dr. Luna currently serves as the lead embryologist at the Equine Embryo Laboratory, performing clinical ICSI as well as continuing to perform research on intracytoplasmic sperm injection, embryo culture, and nuclear transfer.

Brom-de-Luna JG and Hinrichs K. Maturation, ICSI and embryo development as a tool for the equine industry. In:  Equine Assisted Reproduction I, L. Losinno and T. Stout, eds. (in press).

Brom-de-Luna JG, Canesin HS, Wright GA and Hinrichs K. Culture of somatic cells isolated from frozen-thawed equine semen using fluorescence-assisted cell sorting. Anim. Reprod. Sci. 190:10-17 (2018a).

Brom-de-Luna JG, Salgado RM, Canesin HS, Diaw M and Hinrichs K. Effect of incubation temperature and of CO2 concentration during early cleavage on equine in vitro embryo production (Abstract). Reprod. Fertil. Dev. (2019).

Salgado R, Brom-de-Luna JG, Resende HL, Canesin HS, and Hinrichs K. Lower blastocyst quality after conventional vs. Piezo ICSI in the horse reflects delayed sperm component remodeling and oocyte activation. J. Assist. Reprod. Genet. 35:825-840 (2018).

Hélène Resende, DVM, MSHélène Resende, DVM, MS

Research Assistant

Dr. Hélène Resende graduated with a DVM in 2011 from the Federal Rural University of Rio de Janeiro, Brazil. In 2014, she received her Masters in Animal Reproduction at São Paulo State University (UNESP Botucatu), Brazil, with research focused on ovarian and uterine blood flow in young and old mares. She worked as an equine practitioner in Egypt and Belgium before joining the Equine Embryo Laboratory at Texas A&M University in November 2016 as a Research Assistant. Dr. Resende is working on the role of reactive oxygen species in stallion sperm capacitation, as well as on transvaginal follicle aspiration and oocyte manipulation in the mare.