Renato micromanip 2017 final

Publications in referred journals: Reports of original research (2007-2017)


These publications can be viewed online by looking up their title on




Brom-de-Luna JG and Hinrichs K. Maturation, ICSI and embryo development as a tool for the equine industry. In:  Equine Assisted Reproduction I, L. Losinno and T. Stout, eds. (in press).

Brom-de-Luna JG, Salgado RM, Canesin HS, Diaw M and Hinrichs K. Effect of incubation temperature and of CO2 concentration during early cleavage on equine in vitro embryo production (Abstract). Reprod. Fertil. Dev. (2019).

Brom-de-Luna JG, Canesin HS, Wright GA and Hinrichs K. Culture of somatic cells isolated from frozen-thawed equine semen using fluorescence-assisted cell sorting. Anim. Reprod. Sci. 190:10-17 (2018a).

Salgado R, Brom-de-Luna JG, Resende HL, Canesin HS, and Hinrichs K. Lower blastocyst quality after conventional vs. Piezo ICSI in the horse reflects delayed sperm component remodeling and oocyte activation. J. Assist. Reprod. Genet. 35:825-840 (2018).

Valenzuela OA, Couturier-Tarrade A, Choi YH, Aubriére MC, Ritthaler J, Chavatte-Palmer P, and Hinrichs K. Impact of equine assisted reproductive technologies (standard embryo transfer or ICSI/in vitro culture/embryo transfer) on placenta and foal morphometry and placental gene expression. Reprod. Fertil. Dev. Reprod. Fertil. Dev. 30(2):371-379 (2018).

Canesin HS, Brom-de-Luna JG, Choi YH, Ortiz I, Diaw M and Hinrichs K. Blastocyst development after intracytoplasmic sperm injection of equine oocytes vitrified at the germinal-vesicle stage.  Cryobiology 75:52-59 (2017).

Choi YH and Hinrichs K.  Vitrification of in vitro-produced and in vivo-recovered equine blastocysts in a clinical program.  Theriogenology 87:48-54 (2017).

Choi YH, Gibbons JR, Canesin HS and Hinrichs K. Effect of medium variations (zinc supplementation during oocyte maturation, peri-fertilization pH, and embryo culture protein source) on equine embryo development after intracytoplasmic sperm injection. Theriogenology 86:1782-1788 (2016).

Choi YH, Penedo MCT, Daftari P, Velez IC and Hinrichs K. Accuracy of preimplantation genetic diagnosis in equine in vivo-recovered and in vitro-produced blastocysts.  Reprod. Fertil. Dev. 28:1382-1389 (2016).

Pozor MA, Sheppard B, Hinrichs K, Kelleman AA, Macpherson ML, Runcan E, Choi YH, Diaw M and Mathews PM. Placental abnormalities in equine pregnancies generated by somatic cell nuclear transfer from one donor horse. Theriogenology 86:1573-1582 (2016).

Choi YH, Velez IC, Macías-García B, Riera FL, Ballard CS, and Hinrichs K. Effect of clinically-related factors on in vitro blastocyst development after equine ICSI.  Theriogenology 85:1289-1296 (2016).

Lewis N, Hinrichs K, Schnauffer K, Morganti M and Argo C McG. Effect of oocyte source and transport time on rates of equine oocyte maturation and cleavage after fertilisation by ICSI, with a note on the validation of equine embryo morphological classification. Clinical Theriogenology 8: 25-39 (2016).

Riera FL, Roldán JE, Gomez J and Hinrichs K.  Factors affecting the efficiency of foal production in a commercial oocyte transfer program.  Theriogenology 85:1053-1062 (2016).

Choi YH, Ross P, Velez IC, Macías-García B, Riera F and Hinrichs K. Cell lineage allocation in equine blastocysts cultured in varying glucose concentrations. Reproduction 150:31-41 (2015).

Ing NH, Brinsko SP, Curley Jr KO, Forrest DW, Love CC, Hinrichs K, Vogelsang MM, Varner DD and Welsh Jr TH.  Dexamethasone acutely regulates endocrine parameters in stallions and subsequently affects gene expression in testicular germ cells. Anim. Reprod. Sci. 152:47-54 (2015).

Choi YH, Velez IC, Macías-García B and Hinrichs K. Timing factors affecting blastocyst development in equine somatic cell nuclear transfer. Cell. Reprogram. 17:124-130 (2015).

Loux SC, Macías-García B, González-Fernández L, DeSiqueira Canesin H, Varner DD and Hinrichs K. Regulation of axonemal motility in demembranated equine sperm. Biol. Reprod. 91:152, 1-16 (2014).

Macías-García B, González-Fernández L, Rocha AM, Guimarães T, Peña FJ, Varner DD and Hinrichs K. Effect of calcium, bicarbonate and albumin on capacitation-related events in equine sperm.  Reproduction 149:87-99 (2014).

Hayden SS, Blanchard TL, Brinsko SP, Varner DD, Hinrichs K and Love CC.  The “dilution effect” in stallion sperm. Theriogenology  83:772-777 (2014).

Martino NA, Dell’Aquila ME, Filioli Uranio MF, Rutigliano L, Nicassio M, Lacalandra GM and Hinrichs K. Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential. Reprod. Biol. Endocrin. 12:99, 1-12 (2014).

Whigham AR, Blanchard TL, Love CC, Teague SR, Brinsko SP, Welsh Jr TH, Hinrichs K, Varner DD. Equine semen quality following sperm exposure to seminal plasma stored under different conditions. Clinical Theriogenology 6: 459-466 (2014).

Choi YH, Ritthaler J and Hinrichs K.  Production of a mitochondrial-DNA identical cloned foal using oocytes recovered from immature follicles of selected mares. Theriogenology 82:411-417 (2014).

Loux SC, Crawford KR, Ing NH, González-Fernández L, Macías-García B, Love CC, Varner DD, Velez IC, Choi YH and Hinrichs K. CatSper and the relationship of hyperactivated motility to intracellular calcium and pH kinetics in equine sperm. Biol. Reprod. 89:123, 1-15 (2013).

Foss R, Ortis H and Hinrichs K. Effect of potential oocyte transport protocols on blastocyst rates after ICSI in the horse. Equine Vet. J. 45, Suppl. 45:39-43 (2013).

González-Fernández L, Macías-García B, Loux SC, Varner DD, Hinrichs K.  Focal adhesion kinases and calcium/calmodulin-dependent protein kinases regulate protein tyrosine phosphorylation in stallion sperm. Biol. Reprod. 88:138, 1-12 (2013).

Vernunft A, Alm H, Tuchscherer A, Kanitz W, Hinrichs K and Torner H. Chromatin and cytoplasmic characteristics of equine oocytes recovered by transvaginal ultrasound-guided follicle aspiration are influenced by the developmental stage of their follicle of origin.  Theriogenology 80:1-9 (2013).

Choi YH, Norris JD, Velez IC, Jacobson CC, Hartman DL and Hinrichs K A viable foal obtained by equine somatic cell nuclear transfer using oocytes recovered from immature follicles of live mares. Theriogenology 79: 791-796 (2013).

Hinrichs K, Choi YH, Norris JD, Love LB, Bedford-Guaus SJ, Hartman DL and Velez IC. Evaluation of foal production following intracytoplasmic sperm injection and blastocyst culture of oocytes from ovaries collected immediately before euthanasia or after death of mares under field conditions. J. Am. Vet. Med. Assoc. 241:1070-1074 (2012).

Velez IC, Arnold C, Jacobson CC, Norris JD, Choi YH, Edwards JF, Hayden SS and Hinrichs K.  Effects of repeated transvaginal aspiration of immature follicles on mare health and ovarian status. Equine Vet. J. 44, Suppl 43:78-83 (2012).

González-Fernández L, Macías-García B, Velez IC, Varner DD, Hinrichs K.  Calcium-calmodulin and pH regulate protein tyrosine phosphorylation in stallion spermatozoa. Reproduction 144:411-422 (2012).

Cavinder CA, Burns SA, Coverdale JA, Hammer CJ Holub G and Hinrichs K.  Late gestational nutrition of the mare and potential effects on endocrine profiles and adrenal function of the offspring. Prof. Anim. Scientist 28:344-350 (2012).

Hayden SS, Blanchard TL, Brinsko SP, Varner DD, Hinrichs K and Love CC.  Pregnancy rates in mares inseminated with 0.5 or 1 x 106 sperm using hysteroscopic or transrectally guided deep-horn insemination techniques. Theriogenology 78:914-920 (2012).

Choi YH, Varner DD, Love CC, Hartman DL and Hinrichs K.  Production of live foals via intracytoplasmic injection  of lyophilized sperm and sperm extract in the horse.  Reproduction, 142:529-538 (2011).

Foster ML, Love CC, Varner DD, Brinsko SP, Hinrichs K, Teague S, LaCaze K, and Blanchard TL. Comparison of methods for assessing integrity of equine sperm membranes. Theriogenology 76:334-341 (2011).

Choi YH, Velez IC, Riera FL, Roldán JE, Hartman DL, Bliss SB, Blanchard TL, Hayden SS and Hinrichs K. Successful cryopreservation of expanded equine blastocysts. Theriogenology 76:143-152 (2011).

Foster ML, Varner DD, Hinrichs K, Teague S, LaCaze K, Brinsko SP, Blanchard TL and Love CC.  Agreement between measures of total motility and membrane integrity in stallion sperm.  Theriogenology 75:1499-1505 (2011).

Choi YH, Gustafson-Seabury A, Velez IC, Hartman DL, Bliss S, Riera FL Roldán JE, Chowdhary B and Hinrichs K.  Viability of equine embryos after puncture of the capsule and biopsy for preimplantation genetic diagnosis. Reproduction, 140:893-902 (2010).

Mortensen CJ, Choi YH, Ing NH, Kraemer DC, Vogelsang MM and Hinrichs K.  Heat shock protein 70 gene expression in equine blastocysts after exposure of oocytes to high temperatures in vitro or in vivo after exercise of donor mares. Theriogenology 74:374-383 (2010).

Johnson AK, Clark-Price SC, Choi YH, Hartman DL, and Hinrichs K.  Physical and clinicopathologic findings in foals produced by somatic cell nuclear transfer – 14 cases (2005-2008).  J. Am. Vet. Med. Assoc. 236:983-990 (2010).

Jacobson CC, Choi YH, Hayden SS and Hinrichs K.  Recovery of mare oocytes on a fixed biweekly schedule, and resulting blastocyst formation after intracytoplasmic sperm injection.  Theriogenology 73:1116-1126 (2010).

Choi YH, Harding HD, Obermiller AD, Hartman DL, Kurosaka S, McLaughlin KJ, and Hinrichs K.  The uterine environment modulates trophectodermal POU5F1 levels in equine blastocysts. Reproduction  38: 589-599 (2009).

Choi YH, Hartman DL, Fissore RA, Bedford-Guaus SJ and Hinrichs K.  Effect of sperm extract injection volume, injection of PLCζ cRNA, and tissue cell line on efficiency of equine nuclear transfer.  Cloning Stem Cells  11: 301-308 (2009).

McPartlin LA, Suarez SS, Czaya CA, Hinrichs K and Bedford-Guaus SJ.  Hyperactivation of stallion sperm is required for successful in vitro fertilization of equine oocytes. Biol. Reprod. 81:199-206 (2009).

Mortensen CJ, Choi YH, Hinrichs K, Ing NH, Kraemer DC, Vogelsang SG and Vogelsang MM.  Embryo  recovery from exercised mares.  Anim. Reprod. Sci.110:237-244 (2009).

Bedford-Guaus SJ, Yoon S-Y, Fissore RA, Choi Y-H and Hinrichs K. Microinjection of mouse PLC complementary RNA into mare oocytes induces long-lasting intracellular calcium ([Ca2]i) oscillations and embryonic development.  Reprod. Fertil. Dev. 20:875-883 (2008).

Alm H, Choi YH, Love L, Heleil B, Torner H and Hinrichs K.  Holding bovine oocytes in the absence of maturation inhibitors: kinetics of in vitro maturation and effect on blastocyst development after in vitro fertilization. Theriogenology  70:1024-1029 (2008).

Ribeiro BI, Love LB, Choi YH and Hinrichs K. Transport of equine ovaries for assisted reproduction.  Anim. Reprod. Sci.108:171-179 (2008).

Hanna C, Long C, Hinrichs K, Westhusin M and Kraemer D.  Assessment of canine oocyte viability after transportation and storage under different conditions. Anim. Reprod. Sci. 105:451-456 (2008).

Hinrichs K, Choi YH, Varner DD and Hartman DL.  Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin.  Reproduction 134:319-325 (2007).

Hinrichs K, Choi YH, Walckenaer BE, Varner DD, and Hartman DL. In vitro-produced equine embryos: production of foals after transfer to recipient mares, assessment by differential staining, and effect of medium calcium concentrations during culture. Theriogenology  68:521–529 (2007).

Roasa LM, Choi YH, Love CC, Romo S, Varner DD and Hinrichs K. Ejaculate and type of freezing extender affect rates of fertilization of horse oocytes in vitro.  Theriogenology 68:560–566 (2007).

Choi YH, Love LB, Varner DD and Hinrichs K.  Effect of holding technique and culture drop size in individual or group culture on blastocyst development after ICSI of equine oocytes with low meiotic competence.  Animal Reprod. Sci., 102:38-47 (2007).


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