Research

seven publication covers featuring images from the IAL

The Image Analysis Laboratory (IAL) at the Texas A&M College of Veterinary Medicine & Biomedical Sciences (CVMBS) supports the microscopy needs of basic and clinical scientists and their trainees to carry out research to improve the health of animals, humans, and the environment that is consistent with the One Health Initiative of the Texas A&M University System.

Through this initiative, the CVMBS IAL engages in interdisciplinary collaborations with numerous colleges and interdisciplinary programs. 

Listed below are some of the Laboratory’s scholarly contributions that include journal covers featuring images acquired at the IAL and representative recent publications from IAL users.

We appreciate acknowledgment and notification of publications that involved the use of IAL so we can demonstrate productivity for funding of grant applications. We ask users of the laboratory to email us with publications that they would like to be listed.


Selected Publications

Pearson SJ, Roy Sarkar T, McQueen CM, Elswood J, Schmitt EE, Wall SW, Scribner KC, Wyatt G, Barhoumi R, Behbod F, Rijnkels M, Porter WW. ATM-dependent activation of SIM2s regulates homologous recombination and epithelial-mesenchymal transition. Oncogene. 2019 Apr;38(14):2611-2626. doi: 10.1038/s41388-018-0622-4. Epub 2018 Dec 10. PubMed PMID: 30531838; PubMed Central PMCID: PMC6450754.

Peña B, Saha S, Barhoumi R, Burghardt RC, Dunbar KR. Ruthenium(II)-Polypyridyl Compounds with π-Extended Nitrogen Donor Ligands Induce Apoptosis in Human Lung Adenocarcinoma (A549) Cells by Triggering Caspase-3/7 Pathway. Inorg Chem. 2018 Oct 15;57(20):12777-12786. doi: 10.1021/acs.inorgchem.8b01988. Epub 2018 Sep 21. PubMed PMID: 30239197.

Fuentes NR, Mlih M, Barhoumi R, Fan YY, Hardin P, Steele TJ, Behmer S, Prior IA, Karpac J, Chapkin RS. Long-Chain n-3 Fatty Acids Attenuate Oncogenic KRas-Driven Proliferation by Altering Plasma Membrane Nanoscale Pr oteolipid Composition. Cancer Res. 2018 Jul 15;78(14):3899-3912. doi: 10.1158/0008-5472.CAN-18-0324. Epub 2018 May 16. PubMed PMID: 29769200; PubMed Central PMCID: PMC6050089.

Fan YY, Fuentes NR, Hou TY, Barhoumi R, Li XC, Deutz NEP, Engelen MPKJ, McMurray DN, Chapkin RS. Remodelling of primary human CD4+ T cell plasma membrane order by n-3 PUFA. Br J Nutr. 2018 Jan;119(2):163-175. doi: 10.1017/S0007114517003385. Epub 2017 Dec 18. PubMed PMID: 29249211; PubMed Central PMCID: PMC5927572.

Morris DC, Popp JL, Tang LK, Gibbs HC, Schmitt E, Chaki SP, Bywaters BC, Yeh AT, Porter WW, Burghardt RC, Barhoumi R, Rivera GM. Nck deficiency is associated with delayed breast carcinoma progression and reduced metastasis. Mol Biol Cell. 2017 Nov 15;28(24):3500-3516. doi: 10.1091/mbc.E17-02-0106. Epub 2017 Sep 27. PubMed PMID: 28954862; PubMed Central PMCID: PMC5683761.

Schmitt EE, Barhoumi R, Metz RP, Porter WW. Circadian Regulation of Benzo[a]Pyrene Metabolism and DNA Adduct Formation in Breast Cells and the Mouse Mammary Gland. Mol Pharmacol. 2017 Mar;91(3):178-188. doi: 10.1124/mol.116.106740. Epub 2016 Dec 22. PubMed PMID: 28007926; PubMed Central PMCID: PMC5325081.

Hou TY, Barhoumi R, Fan YY, Rivera GM, Hannoush RN, McMurray DN, Chapkin RS. n-3 polyunsaturated fatty acids suppress CD4(+) T cell proliferation by altering phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] organization. Biochim Biophys Acta. 2016 Jan;1858(1):85-96. doi: 10.1016/j.bbamem.2015.10.009. Epub 2015 Oct 23. PubMed PMID: 26476105; PubMed Central PMCID: PMC4663135.

Bazer FW, Burghardt RC, Johnson GA, Spencer TE, Wu G. Mechanisms for the establishment and maintenance of pregnancy: synergies from scientific collaborations. Biol Reprod. 2018 Jul 1;99(1):225-241. doi: 10.1093/biolre/ioy047. PubMed PMID: 29462279; PubMed Central PMCID: PMC6044348.

Steinhauser CB, Wing TT, Gao H, Li X, Burghardt RC, Wu G, Bazer FW, Johnson GA. Identification of appropriate reference genes for qPCR analyses of placental expression of SLC7A3 and induction of SLC5A1 in porcine endometrium. Placenta. 2017 Apr;52:1-9. doi: 10.1016/j.placenta.2017.02.003. Epub 2017 Feb 4. PubMed PMID: 28454689.

Banu SK, Stanley JA, Sivakumar KK, Taylor RJ, Arosh JA, Burghardt RC. Editor’s Highlight: Exposure to CrVI during Early Pregnancy Increases Oxidative Stress and Disrupts the Expression of Antioxidant Proteins in Placental Compartments. Toxicol Sci. 2017 Feb 1;155(2):497-511. doi: 10.1093/toxsci/kfw231r. PubMed PMID: 28427089.

Frank JW, Seo H, Burghardt RC, Bayless KJ, Johnson GA. ITGAV (alpha v integrins) bind SPP1 (osteopontin) to support trophoblast cell adhesion. Reproduction. 2017 May;153(5):695-706. doi: 10.1530/REP-17-0043. Epub 2017 Mar 1. PubMed PMID: 28250242.

Banu SK, Stanley JA, Sivakumar KK, Taylor RJ, Arosh JA, Burghardt RC. Editor’s Highlight: Exposure to CrVI during Early Pregnancy Increases Oxidative Stress and Disrupts the Expression of Antioxidant Proteins in Placental Compartments. Toxicol Sci. 2017 Feb;155(2):497-511. doi: 10.1093/toxsci/kfw231. Epub 2016 Nov 15. PubMed PMID: 28077780; PubMed Central PMCID: PMC5291216.

Steinhauser CB, Landers M, Myatt L, Burghardt RC, Vallet JL, Bazer FW, Johnson
GA. Fructose Synthesis and Transport at the Uterine-Placental Interface of Pigs: Cell-Specific Localization of SLC2A5, SLC2A8, and Components of the Polyol Pathway. Biol Reprod. 2016 Nov;95(5):108. Epub 2016 Aug 17. PubMed PMID: 27535960.

Wang X, Johnson GA, Burghardt RC, Wu G, Bazer FW. Uterine Histotroph and Conceptus Development. II. Arginine and Secreted Phosphoprotein 1 Cooperatively Stimulate Migration and Adhesion of Ovine Trophectoderm Cells via Focal Adhesion-MTORC2 Mediated Cytoskeleton Reorganization. Biol Reprod. 2016 Sep;95(3):71. Epub 2016 Aug 10. PubMed PMID: 27512152.

Banu SK, Stanley JA, Sivakumar KK, Arosh JA, Taylor RJ, Burghardt RC. Chromium
VI – Induced developmental toxicity of placenta is mediated through spatiotemporal dysregulation of cell survival and apoptotic proteins. Reprod Toxicol. 2017 Mar;68:171-190. doi: 10.1016/j.reprotox.2016.07.006. Epub 2016 Jul 18. PubMed PMID: 27443218; PubMed Central PMCID: PMC5830189.

Banu SK, Stanley JA, Sivakumar KK, Arosh JA, Burghardt RC. Resveratrol protects the ovary against chromium-toxicity by enhancing endogenous antioxidant enzymes and inhibiting metabolic clearance of estradiol. Toxicol Appl Pharmacol. 2016 Jul 15;303:65-78. doi: 10.1016/j.taap.2016.04.016. Epub 2016 Apr 27. PubMed PMID: 27129868; PubMed Central PMCID: PMC5830085.

Chaki SP, Barhoumi R, Rivera GM. Actin remodeling by Nck regulates endothelial lumen formation. Mol Biol Cell. 2015 Sep 1;26(17):3047-60. doi: 10.1091/mbc.E15-06-0338. Epub 2015 Jul 8. PubMed PMID: 26157164; PubMed Central PMCID: PMC4551318.

Banu SK, Stanley JA, Sivakumar KK, Arosh JA, Barhoumi R, Burghardt RC. Identifying a novel role for X-prolyl aminopeptidase (Xpnpep) 2 in CrVI-induced adverse effects on germ cell nest breakdown and follicle development in rats. Biol Reprod. 2015 Mar;92(3):67. doi: 10.1095/biolreprod.114.125708. Epub 2015 Jan 7. PubMed PMID: 25568306; PubMed Central PMCID: PMC4358023.

Stanley JA, Arosh JA, Burghardt RC, Banu SK. A fetal whole ovarian culture model for the evaluation of CrVI-induced developmental toxicity during germ cell nest breakdown. Toxicol Appl Pharmacol. 2015 Sep 5. pii: S0041-008X(15)30076-4. doi: 10.1016/j.taap.2015.09.002. [Epub ahead of print] PubMed PMID: 26348139.

Arosh JA, Lee J, Balasubbramanian D, Stanley JA, Long CR, Meagher MW, Osteen KG, Bruner-Tran KL, Burghardt RC, Starzinski-Powitz A, Banu SK. Molecular and preclinical basis to inhibit PGE2 receptors EP2 and EP4 as a novel nonsteroidal therapy for endometriosis. Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):9716-21. doi: 10.1073/pnas.1507931112. Epub 2015 Jul 21. PubMed PMID: 26199416; PubMed Central PMCID: PMC4534219.

Ma X, Hou YQ, Dahanayaka S, Satterfield MC, Burghardt RC, Bazer FW, Wu G. Technical note: Isolation and characterization of ovine brown adipocyte precursor cells. J Anim Sci. 2015 May;93(5):2094-9. doi: 10.2527/jas.2014-8728. PubMed PMID: 26020305.

Wang X, Burghardt RC, Romero JJ, Hansen TR, Wu G, Bazer FW. Functional roles of arginine during the peri-implantation period of pregnancy. III. Arginine stimulates proliferation and interferon tau production by ovine trophectoderm cells via nitric oxide and polyamine-TSC2-MTOR signaling pathways. Biol Reprod. 2015 Mar;92(3):75. doi: 10.1095/biolreprod.114.125989. Epub 2015 Feb 4. PubMed PMID: 25653279.

Barhoumi R, Mouneimne Y, Chapkin RS, Burghardt RC. Effects of Fatty Acids on Benzo[a]pyrene Uptake and Metabolism in Human Lung Adenocarcinoma A549 Cells. PLoS One. 2014 Mar 20;9(3):e90908. PubMed PMID: 24651647; PubMed Central PMCID: PMC3961214.

Peña B, Barhoumi R, Burghardt RC, Turro C, Dunbar KR. Confocal fluorescence microscopy studies of a fluorophore-labeled dirhodium compound: visualizing metal-metal bonded molecules in lung cancer (A549) cells. J Am Chem Soc. 2014 May 22. [Epub ahead of print]

Jutooru I, Guthrie AS, Chadalapaka G, Pathi S, Kim K, Burghardt R, Jin UH, Safe S. Mechanism of action of phenethylisothiocyanate and other ROS-inducing anticancer agents. Mol Cell Biol. 2014 Apr 14. [Epub ahead of print]

Laughlin RC, Knodler LA, Barhoumi R, Payne HR, Wu J, Gomez G, Pugh R, Lawhon SD, Bäumler AJ, Steele-Mortimer O, Adams LG. Spatial segregation of virulence gene expression during acute enteric infection with Salmonella enterica serovar Typhimurium. MBio. 2014 Feb 4;5(1):e00946-13. PubMed PMID: 24496791; PubMed Central PMCID: PMC3950517.

Kim W, Barhoumi R, McMurray DN, Chapkin RS. Dietary fish oil and DHA down-regulate antigen-activated CD4+ T-cells while promoting the formation of liquid-ordered mesodomains. Br J Nutr. 2014 Jan 28;111(2):254-60PubMed PMID: 23962659.

Li X, Bazer FW, Johnson GA, Burghardt RC, Frank JW, Dai Z, Wang J, Wu Z, Shinzato I, Wu G. Dietary supplementation with L-arginine between days 14 and 25 of gestation enhances embryonic development and survival in gilts. Amino Acids. 2014 Feb;46(2):375-84. PubMed PMID: 24337831.

Sivakumar KK, Stanley JA, Arosh JA, Pepling ME, Burghardt RC, Banu SK. Prenatal exposure to chromium induces early reproductive senescence by increasing germ cell apoptosis and advancing germ cell cyst breakdown in the F1 offspring. Dev Biol. 2014 Apr 1;388(1):22-34. PubMed PMID: 24530425.

Wang X, Frank JW, Little DR, Dunlap KA, Carey Satterfield M, Burghardt RC,Hansen TR, Wu G, Bazer FW. Functional role of arginine during the peri-implantation period of pregnancy. I. Consequences of loss of function of arginine transporter SLC7A1 mRNA in ovine conceptus trophectoderm. FASEB J. 2014 Mar 13. PubMed PMID: 24627544.

Li F, Yu X, Szynkarski CK, Meng C, Zhou B, Barhoumi R, White RE, Heaps CL, Stallone JN, Han G. Activation of GPER Induces Differentiation and Inhibition of Coronary Artery Smooth Muscle Cell Proliferation. PLoS One. 2013 Jun 19;8(6):e64771. PubMed PMID: 23840305; PubMed Central PMCID: PMC3686788.

Chaki SP, Barhoumi R, Berginski ME, Sreenivasappa H, Trache A, Gomez SM, Rivera GM. Nck enables directional cell migration through the coordination of polarized membrane protrusion with adhesion dynamics. J Cell Sci. 2013 Apr 1;126(Pt 7):1637-49. PubMed PMID: 23444376.

Streifel KM, Miller J, Mouneimne R, Tjalkens RB. Manganese inhibits ATP-induced calcium entry through the transient receptor potential channel TRPC3 in astrocytes. Neurotoxicology. 2013 Jan;34:160-6. PubMed PMID: 23131343; PubMed Central PMCID: PMC3557543.

Oelusarz A, Nichols LA, Grunz-Borgmann EA, Chen G, Akintola AD, Catania JM, Burghardt RC, Trzeciakowski JP, Parrish AR. Overexpression of MMP-7 increases collagen 1A2 in the aging kidney. Physiol Rep. 2013 Oct;1(5). pii: e00090.

Kim K, Jutooru I, Chadalapaka G, Johnson G, Frank J, Burghardt R, Kim S, Safe S. HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer. Oncogene. 2013 Mar 28;32(13):1616-25.

Baker LK, Gillis DC, Sharma S, Ambrus A, Herrmann H, Conover G. Nebulin binding impedes mutant desmin filament assembly. Molecular Biology of the Cell. 2013;24:1918-32. PubMed PMID: 23615443.

Storey SM, McIntosh AL, Huang H, Martin GG, Landrock KK, Landrock D, Payne HR, Kier AB, Schroeder F. Loss of Intracellular Lipid Binding Proteins Differentially Impacts Saturated Fatty Acid Uptake and Nuclear Targeting in Mouse Hepatocyes. American journal of physiology Gastrointestinal and liver physiology. 2012;303:G837-G50. PubMed PMID: 22859366; PubMed Central PMCID: PMC3469595.

Storey SM, McIntosh AL, Huang H, Landrock KK, Martin GG, Landrock D, Payne HR, Atshaves BP, Kier AB, Schroeder F. Intracellular Cholesterol Binding Proteins Enhance HDL-mediated Cholesterol Uptake in Cultured Primary Mouse Hepatocytes. American journal of physiology Gastrointestinal and liver physiology. 2012. Epub Jan 12, 2012. PubMed PMID: 22241858; PubMed Central PMCID: PMC3355564.

Turk HF, Barhoumi R, Chapkin RS. Alteration of EGFR spatiotemporal dynamics suppresses signal transduction. PLoS One. 2012;7(6):e39682. PubMed PMID: 22761867; PubMed Central PMCID: PMC3384615.

Steelman AJ, Thompson JP, Li J. Demyelination and remyelination in anatomically distinct regions of the corpus callosum following cuprizone intoxication. Neuroscience Research.2012;72:32-42. PubMed PMID: 22015947.

Stoica G, Lungu G, Bjorklund NL, Taglialatela G, Zhang X, Chiu V, Hill HH, Schenk JO, Murray I. Potential role of α-synuclein in neurodegeneration: studies in a rat animal model. J Neurochem.2012;122:812-22. PubMed PMID: 22639889.

Hou TY, Monk JM, Fan YY, Barhoumi R, Chen YQ, Rivera GM, McMurray DN, Chapkin RS. n-3 polyunsaturated fatty acids suppress phosphatidylinositol 4,5-bisphosphate-dependent actin remodeling during CD4+ T-cell activation. Biochem J. 2012 Apr 1;443(1):27-37. PubMed PMID: 22250985; PubMed Central PMCID: PMC3814172.

Kamkaew A, Barhoumi R, Burghardt RC, Burgess K. Cationic polyfluorenes for intracellular delivery of proteins. Org Biomol Chem. 2011 Oct 7;9(19):6513-8. PubMed PMID: 21845277; PubMed Central PMCID: PMC3433840.

Tiffany-Castiglioni E, Qian Y, Barhoumi R, Zheng Y. Determination of metal interactions with the chaperone Hspa5 in human astrocytoma cells and rat astrocyte primary cultures. Methods Mol Biol. 2011;758:29-48. PubMed PMID: 21815057.

Ibrahim A, El-Meligy A, Lungu G, Fetaih H, Dessouki A, Stoica G, Barhoumi R. Curcumin induces apoptosis in a murine mammary gland adenocarcinoma cell line through the mitochondrial pathway. Eur J Pharmacol. 2011 Oct 1;668(1-2):127-32. PubMed PMID: 21762689.

Kolar S, Barhoumi R, Jones CK, Wesley J, Lupton JR, Fan YY, Chapkin RS. Interactive effects of fatty acid and butyrate-induced mitochondrial Ca²⁺ loading and apoptosis in colonocytes. Cancer. 2011 Dec 1;117(23):5294-303. PubMed PMID: 21563175; PubMed Central PMCID: PMC3156959.

Jose J, Loudet A, Ueno Y, Wu L, Chen HY, Son DH, Barhoumi R, Burghardt R, Burgess K. Energy transfer cassettes in silica nanoparticles target intracellular organelles. Org Biomol Chem. 2011 May 21;9(10):3871-7. PubMed PMID: 21455504.

Barhoumi R, Mouneimne Y, Ramos E, Morisseau C, Hammock BD, Safe S, Parrish AR, Burghardt RC. Multiphoton spectral analysis of benzo[a]pyrene uptake and metabolism in a rat liver cell line. Toxicol Appl Pharmacol. 2011 May 15;253(1):45-56. PubMed PMID: 21420996; PubMed Central PMCID: PMC3081912.

Storey SM, Atshaves BP, McIntosh AL, Landrock D, Martin GG, Huang H, Payne HR, Johnson JD, Macfarlane RD, Kier AB, Schroeder F. Effect of sterol carrier protein-2 gene ablation on HDL-mediated cholesterol efflux from cultured primary mouse hepatocytes. American journal of physiology Gastrointestinal and liver physiology. 2010;299:G244-G54. PubMed PMID: 20395534; PubMed Central PMCID: PMC2904118.

Porter BF, Ridpath JF, Calise DV, Payne HR, Janke JJ, Baxter DG, Edwards JF. Hypomyelination associated with bovine viral diarrhea virus type 2 infection in a longhorn calf. Vet Pathol.2010;47(4):658-63. PubMed PMID: 20448278.

Hoppes S, Gray PL, Payne S, Shivaprasad HL, Tizard I. The Isolation, pathogenesis, diagnosis, transmission and control of Avian Bornavirus and proventricular dilatation disease. Veterinary Clinics of North America Exotic Animal Practice. 2010;13:495-508. PubMed PMID: 20682432.

Maheshwari M, Roberts JK, DeSutter B, Duong K, Tingling J, Fawver JN, Schall HE, Kahle M, Murray IVJ. Hydralazine modifies Aß fibril formation and prevents modification by lipids in vitro. Biochemistry. 2010;49:10371-80. PubMed PMID: 21058733.

Ibrahim A, El-Meligy A, Fetaih H, Dessouki A, Stoica G, Barhoumi R. Effect of curcumin and Meriva on the lung metastasis of murine mammary gland adenocarcinoma. In Vivo. 2010 Jul-Aug;24(4):401-8. PubMed PMID: 20668306.

Vanderlaag K, Su Y, Frankel AE, Burghardt RC, Barhoumi R, Chadalapaka G, Jutooru I, Safe S. 1,1-Bis(3′-indolyl)-1-(p-substituted phenyl)methanes induce autophagic cell death in estrogen receptor negative breast cancer. BMC Cancer. 2010 Dec 3;10:669. PubMed PMID: 21129193; PubMed Central PMCID: PMC3003661.