Charles Long
Professor Department of Veterinary Physiology & PharmacologyResearch and Scholarly Interests
Gamete and embryo physiology, epigenetics, assisted reproductive technologies, genetic engineering of livestock and companion animals, biomedical models of human disease, disease resistant livestock
Education
- Post Doctoral Training Gamete and Embryo Physiology, ARS-USDA 1998
- PHD Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 1996
- MS Department of Animal Science, University of Missouri, Columbia 1989
- BS Department of Animal Science, University of Missouri, Columbia 1986
I have conducted research on the physiology of gametes and early embryos for over 25 years in both private industry and government laboratories. These studies have included nuclear reprogramming in cloned embryos, in vitro fertilization systems using sex sorted sperm, embryo cryobiology, cytoskeletal and nuclear abnormalities of in vitro produced livestock embryos as well as microtubule nucleating components of sperm during zygotic development.
- Cell Biology
- Genetics
- Reproductive and Developmental Biology
My more recent research interests have focused on the role of histone and DNA methyltransferase genes in the control of epigenetic reprogramming during early bovine embryonic development. We are specifically interested in the exogenous stress that in vitro culture of embryos places on the epigenome and how these stresses manifest in altered or failed fetal development.
My laboratory also investigates novel transgenic approaches to enhance production and disease resistance traits in livestock and companion animals. For example, Dr. Mark Westhusin and I have ongoing collaborations to produce biologically active proteins in the milk of transgenic animals. Currently we maintain goats that produce antigens in their milk that can be purified and delivered as vaccines. We have ongoing projects utilizing RNA interference and gene editing technologies to enhance production or fight a number of economically important livestock and human diseases. Furthermore, we utilize these same genetic engineering strategies to produce biomedical models of human disease. Current projects are focused on development of inducible, tissue specific expression of transgenes to produce a model of human obesity and metabolic syndrome in swine.
Many of the projects are accomplished in collaboration with Drs. Golding, Westhusin and Kraemer of the Reproductive Sciences Laboratory. This is dynamic group that offers a unique and stimulating environment for conducting research. In addition we maintain active collaborations with a number of other laboratories in academia, government and private industry.
My courses all focus on embryology and reproductive physiology.
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Charles R. Long is not accepting trainees at this time.
Oxygen-induced alterations in the expression of chromatin modifying enzymes and the transcriptional regulation of imprinted genes (2018) Gene Expression Patterns 10.1016/j.gep.2018.01.001
DNA methylation-independent growth restriction and altered developmental programming in a mouse model of preconception male alcohol exposure (2017) Epigenetics 10.1080/15592294.2017.1363952
Depletion of elongation initiation factor 4E binding proteins by CRISPR/Cas9 enhances the antiviral response in porcine cells (2016) Antiviral Research 10.1016/j.antiviral.2015.11.002
Inhibition of EHMT2 Induces a Robust Antiviral Response Against Foot-and-Mouth Disease and Vesicular Stomatitis Virus Infections in Bovine Cells (2016) Journal of Interferon and Cytokine Research 10.1089/jir.2015.0006
Genome edited sheep and cattle (2015) Transgenic Research 10.1007/s11248-014-9832-x
Histone-lysine N-methyltransferase SETDB1 is required for development of the bovine blastocyst (2015) Theriogenology 10.1016/j.theriogenology.2015.07.028
Molecular and preclinical basis to inhibit PGE2 receptors EP2 and EP4 as a novel nonsteroidal therapy for endometriosis (2015) Proceedings of the National Academy of Sciences of the United States of America 10.1073/pnas.1507931112
Expression of porcine fusion protein IRF7/3(5D) efficiently controls foot-and-mouth disease virus replication (2014) Journal of Virology 10.1128/JVI.00372-14
Reshaping the transcriptional frontier: Epigenetics and somatic cell nuclear transfer (2014) Molecular Reproduction and Development 10.1002/mrd.22271
Sustained expression of insulin by a genetically engineered sertoli cell line after allotransplantation in diabetic BALB/c mice (2014) Biology of Reproduction 10.1095/biolreprod.113.115600
Transgenic sheep generated by lentiviral vectors: Safety and integration analysis of surrogates and their offspring (2013) Transgenic Research 10.1007/s11248-012-9674-3
Down-regulation of viral replication by lentiviral-mediated expression of short-hairpin RNAs against vesicular stomatitis virus ribonuclear complex genes (2012) Antiviral Research 10.1016/j.antiviral.2012.05.007
Efficient TALEN-mediated gene knockout in livestock (2012) Proceedings of the National Academy of Sciences of the United States of America 10.1073/pnas.1211446109
Production of transgenic calves expressing an shRNA targeting myostatin (2012) Molecular Reproduction and Development 10.1002/mrd.22007
Cryopreservation of in vitro produced bovine embryos: Effects of lipid segregation and post-thaw laser assisted hatching (2011) Theriogenology 10.1016/j.theriogenology.2010.07.006
Examination of DNA methyltransferase expression in cloned embryos reveals an essential role for Dnmt1 in bovine development (2011) Molecular Reproduction and Development 10.1002/mrd.21306
Applications of RNA interference-based gene silencing in animal agriculture (2010) Reproduction, Fertility and Development 10.1071/RD09211
Clinical and molecular characterization of a re-established line of sheep exhibiting hemophilia A (2010) Journal of Thrombosis and Haemostasis 10.1111/j.1538-7836.2009.03697.x
Embryo production and possible species preservation by nuclear transfer of somatic cells isolated from bovine semen (2010) Theriogenology 10.1016/j.theriogenology.2010.06.035
Viral particles of endogenous betaretroviruses are released in the sheep uterus and infect the conceptus trophectoderm in a transspecies embryo transfer model (2010) Journal of Virology 10.1128/JVI.00950-10
Evaluation of culture systems for attachment and proliferation of epithelial cells cultured from ovine semen (2009) Animal Reproduction Science 10.1016/j.anireprosci.2008.11.012
Assessment of canine oocyte viability after transportation and storage under different conditions (2008) Animal Reproduction Science 10.1016/j.anireprosci.2007.12.009
Synchronisation of canine germinal vesicle stage oocytes prior to in vitro maturation alters the kinetics of nuclear progression during subsequent resumption of meiosis (2008) Reproduction, Fertility and Development 10.1071/RD07227
Identification, amplification and characterization of miR-17-92 from canine tissue (2007) Gene 10.1016/j.gene.2007.08.015
Suppression of prion protein in livestock by RNA interference (2006) Proceedings of the National Academy of Sciences of the United States of America 10.1073/pnas.0600813103
Epigenetic and genomic imprinting analysis in nuclear transfer derived Bos gaurus/Bos taurus hybrid fetuses (2004) Biology of Reproduction 10.1095/biolreprod.103.025775
New commercial opportunities for advanced reproductive technologies in horses, wildlife, and companion animals (2003) Theriogenology 10.1016/S0093-691X(02)01266-9
Cloning to reproduce desired genotypes (2001) Theriogenology 10.1016/S0093-691X(00)00444-1
Birth of piglets after transfer of embryos cryopreserved by cytoskeletal stabilization and vitrification (2000) Biology of Reproduction 10.1095/biolreprod62.3.564
Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells (2000) Biology of Reproduction 10.1095/biolreprod62.5.1135
Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses (2000) Biology of Reproduction 10.1095/biolreprod62.5.1135
Correlation between Histochemically Assessed Fiber Type Distribution and Isomyosin and Myosin Heavy Chain Content in Porcine Skeletal Muscles (1999) Journal of Animal Science 10.2527/1999.7782104x
In vitro production of pig embryos: Comparisons of culture media and boars (1999) Theriogenology 10.1016/S0093-691X(99)00081-3
In vitro production of sexed embryos for gender preselection: High-speed sorting of X-chromosome-bearing sperm to produce pigs after embryo transfer (1999) Journal of Animal Science 10.2527/1999.77123346x
Initiation and organization of events during the first cell cycle in mammals: Applications in cloning (1999) Cloning 10.1089/15204559950019979
Review: Initiation and organization of events during the first cell cycle in mammals: Applications in cloning (1999) Cloning and Stem Cells 10.1089/15204559950019979
Dual labeling of the cytoskeleton and DNA strand breaks in porcine embryos produced in vivo and in vitro (1998) Molecular Reproduction and Development 10.1002/(SICI)1098-2795(199809)51:1<59::AID-MRD7>3.0.CO;2-V
The relationship between membrane status and fertility of boar spermatozoa after flow cytometric sorting in the presence or absence of seminal plasma (1998) Reproduction, Fertility and Development 10.1071/RD98102
Verification of flow cytometorically-sorted X- and Y-bearing porcine spermatozoa and reanalysis of spermatozoa for DNA content using the fluorescence in situ hybridization (FISH) technique (1998) Theriogenology 10.1016/S0093-691X(98)00167-8
Isolation and characterization of MPM-2-reactive sperm proteins: Homology to components of the outer dense fibers and segmented columns (1997) Biology of Reproduction 10.1095/biolreprod57.2.246
Evaluation of developmental competence, nuclear and ooplasmic maturation of calf oocytes (1996) Molecular Reproduction and Development 10.1002/(SICI)1098-2795(199612)45:4<521::AID-MRD15>3.0.CO;2-Z
Factors involved in nuclear reprogramming during early development in the rabbit (1995) Molecular Reproduction and Development 10.1002/mrd.1080400305
Inactivation of histone H1 kinase by Ca2+ in rabbit oocytes (1995) Molecular Reproduction and Development 10.1002/mrd.1080400215
Morphology and subsequent development in culture of bovine oocytes matured in vitro under various conditions of fertilization (1994) Journal of Reproduction and Fertility 10.1002/mrd.1080400215
Chromatin and microtubule morphology during the first cell cycle in bovine zygotes (1993) Molecular Reproduction and Development 10.1002/mrd.1080360105