Individuals desiring to use the Image Analysis Laboratory for
research are expected to comply with the guidelines described
An account number for each use of an instrument must be on file
before the instrumentation is used.
Sign up procedures for electron microscopy instrumentation
Individuals may reserve electron microscopy equipment in person,
by phone (979)845-9527, or by e-mail (firstname.lastname@example.org).
Electron microscopy equipment may be scheduled for up to one week
in advance beginning on Monday morning. The sign-up calendar is
located in room B20. Users who fail to show up within a 15
minute grace period past the time for which they have reserved
electron optical and support equipment will forfeit their scheduled
equipment time. The forfeited period of time is available
on a first-come, first-serve basis to other users. Persons
interested in making use of such forfeited time are encouraged to
first sign in on the scope log and then make necessary changes on
the sign-up board. If any questions or problems are encountered or
if any individuals are in doubt of their status, please contact Dr.
Burghardt (979)862-4083. Individuals who repeatedly fail to show up
for scheduled time will receive an initial written warning, and if
the practice persists, will be billed for the scheduled time. These
policies do not apply to the laser cytometry instruments.
Sign Up Procedures for Fluorescence Instrumentation
Fluorescence instruments other than the Meridian Ultima and
InSIGHT Point may be operated by individual researchers who are
trained and checked out by one of the facility staff for the
particular application desired. Image Analysis staff are currently
responsible for operation of both Ultima and InSIGHT instruments.
Each researcher using fluorescence facilities is responsible for
the design and analysis of his/her experiments. Core facility
personnel may provide experimental design and data analysis support
based on normal collaborative research agreements. All applications
involving the Laser Cytometry are scheduled through Dr. Rola
Barhoumi in person, by phone (979)458-1149, or by e-mail (
email@example.com ). Scheduling any fluorescence imaging
equipment can be done up to, but not exceeding 8 weeks in advance.
Other scheduling needs must be arranged with the Faculty Director.
Researchers should not schedule more time than needed, as charges
will be based on the scheduling log. Users must keep track of the
amount of time the fluorescence facility is used each day. A user
may cancel scheduled time without charge if the cancellation is
done at least 24 hours in advance of the scheduled time.
Unscheduled and unused scheduled times will be available on a
first-come, first-serve basis and are subject to normal fees.
Schedules are subject to rearrangement by the Faculty Director when
needed. The Meridian Ultima and InSIGHT Point may be used one day
per week for facility development and maintenance. Dye loading,
cell manipulations, etc. will be performed in the adjoining cell
culture facility following the rules and guidelines of the
facility. Each researcher is responsible for their own cell
culturing, dye loading, experimental procedures, and for providing
optical disks for data storage. Each researcher's data will be
stored on an optical disk and NOT on the hard disk. The researcher
must provide his/her own disk(s). Any data left on the hard disk at
the end of the week is subject to erasure.
All new electron microscope and major equipment
users will be required to demonstrate competency on the instrument
they propose to operate. Investigators who have qualified on
instruments elsewhere must be instructed in the use of the current
equipment prior to checkout. If further training is required to
demonstrate competency, scheduled sessions will be available and
posted in the Image Analysis Laboratory. Individual operators must
be able to assess the working condition of an instrument and they
will be held responsible for informing Image Analysis Laboratory
personnel if an instrument is not performing properly.
All microscopes will be maintained in a ready state by Image
Analysis Laboratory staff. This ready state of the microscopes will
permit individuals desiring such services to sign-up for
instruments during the normal working week. Individuals failing to
comply with Policies and Procedures will be subject to forfeiture
Use of the instrumentation will require a user's fee that will
be posted annually. Each instrument is intended to provide a
specific function and therefore certain restrictions are stipulated
for instruments. These limitations will be listed in the
instrumentation instruction handouts that are available to Image
Analysis Laboratory users.
Separate charges will be billed for technical staff-assisted
projects outside the supporting departments. Technical services are
limited primarily to microscope operation although certain types of
specimen preparation are available for electron microscopy
applications. Contact prints of micrographs are included in
technical services charges. A Service Request form will be filled
out by the individual requesting service and will include account
information, project description and sample history.
All Image Analysis Laboratory users must have an account number
on file prior to using the Laboratory. Forms for
reporting account numbers are available through the staff. All
instrument time must be logged in the appropriate place with the
users name (not initials), department, account number, date, time
in-out, time used and number of exposures, and comments regarding
the condition of the microscope. Bills will be tabulated and mailed
monthly, stating the status of your account.
The electron beam hours (EBH) charge will
include the following
Items not supplied
- Film (EM-4489)
- Photographic chemistry for film development
- Use of ancillary equipment i.e., microtomes, knife makers,
- Digital scans of negatives.
- Fixatives, buffers, embedding supplies and resins, alcohols,
- Grids, stubs, forceps, Reichert capsules, etc.
- Glass strips for knives, dispo boats, etc.
- Photographic paper, press on letters, art supplies, etc.
- Carbon rods, platinum wire, tungsten wire, etc.
- Optical disks, cuvettes, chamber slides, probes, etc.
All users of the Image Analysis Laboratory are responsible for
the safe utilization of instrumentation. Electrical and chemical
hazards, as well as hazards associated with the utilization of
high-vacuum instruments exist. No biohazardous material may be
brought into the laboratory unless procedures for their use and
disposal have been approved and all members of the Image Analysis
Laboratory are properly informed of potential hazards. A handbook
of safety procedures and a Texas A&M University
Hazardous Waste Disposal Manual is located at the entrance
of the laboratory. Users of the Laboratory are obligated to use
proper safety procedures, and are expected to both read and
understand the contents of these documents. Material Data Safety
Sheets are maintained on all chemicals used in the laboratory.
Contact a staff member for manuals.
Biosafety requirements and rules
Selected rooms in the Image Analysis Laboratory have been
approved as BL-2 laboratory space. In order to be able to bring the
BL-2 material to the IAL, the IAL facility and room number
MUST be listed in the investigator's IBC permit,
in Section F, Agent use and Storage Locations. The investigator is
required to send a copy of the IBC permit listing the
relevant IAL laboratory room and the BL2 organisms, to firstname.lastname@example.org,
before bringing the BL-2 samples. When using the BL-2 labs in the
IAL, all users must follow the rules posted. This applies even to
those users that do not work with samples requiring IBC permit.