Skip to main content
What can we help you find?

Capabilities

Our laboratory is equipped to bridge the gap between in vitro models to in vivo experiments in large animals, including horses and livestock. Our lab is well-equipped with state-of-the-art equipment for measuring and modeling aerosol drug delivery, including delivery to primary respiratory cell culture systems. We work together with the AALAC-accredited Veterinary Medical Park to perform animal studies on-site in large animal species.

We are always looking for opportunities to collaborate – please reach out to Dr. Legere for more information about how our resources could be useful for your projects.

Aerosol Delivery

  • Simulated airways:
    • 3-D printed models to simulate the head and upper airway of large animal speciesUSP throat models
  • Breathing Simulators:
    • Inhalation flow cell & high-capacity pumps to simulate inhalation and capture inhaled aerosols for testing
    • Custom-built negative-pressure ventilation chamber for precision respiratory modeling using ex vivo lungs and lung models
      • Research capacity – Can be used to deliver aerosols to inflated, “breathing” lungs and model customized respiratory parameters in ex vivo tissues
      • Teaching capacity – Can be used to teach respiratory interventions (e.g., endoscopy, airway sampling, etc.) and ventilator operation
  • Particle Size Analysis:
    • Andersen cascade impactor
  • Customizable Filter Systems: To collect aerosolized drugs for delivered dose calculation & further analysis
  • Digital viscometer
  • UV/Visible scanning spectrophotometer: To measure drug concentrations in solution (Shimadzu UV-1800)

Cell Culture

  • Digital fluorescent microscope (Evos M5000)
  • Fluorescent cell counter (Countess 3 FL)
  • Biosafety cabinet for BSL-2 work
  • Warming baths – water-based and dry-bead
  • CO2 incubators for BSL-2 work
  • Custom chambers to enable aerosol drug delivery to culture plates
  • Equipment & expertise for isolating primary lung and guttural pouch epithelial cultures
  • Confocal and specialty imaging through Image Analysis Laboratory

Biosensor Technologies

  • Cardiac telemetry/holter monitors (Televet II) with dedicated tablets and heart rate variability analysis
  • Wearable telemetry with ECG, plethysmography, accelerometer, and specialty signal analysis software (EmkaPack5)
  • Patient monitor with ECG, non-invasive and invasive BP, pulse oximetry, and end-tidal CO2 monitoring (Philips Intellivue)

Please reach out to Dr. Legere for more information on our laboratory capabilities and opportunities to collaborate in research and teaching.