Before scheduling any Image Analysis Laboratory (IAL) equipment, please register in iLabs at https://tamu.corefacilities.org/service_center/show_external/4876.
- For the first-time use of:
- optical microscopy technologies, contact Dr. Robert Burghardt at rburghardt@cvm.tamu.edu or Dr. Saugata Mahapatra at saugata@tamu.edu.
- transmission electron (TEM) microscopy, contact Dr. Joseph Szule at jszule@cvm.tamu.edu.
- Cell Discoverer 7 (CD7), contact Ernesto Ramos at eramos@cvm.tamu.edu.
Zeiss LSM 780 Confocal with Airyscan
- 34 Ch spectral GaAsP detection confocal/multiphoton microscope
- Continuous wavelength laser lines at 405, 458, 488, 514, 543, 561, 594, and 633 nm
- Definite focus, Incubation (Temperature & CO2), and motorized stage
- ZEN Blue software FRET, FRAP, Physiology, and 3D VisArt modules
- Objectives: Plan-Apo 10X/0.45, Plan-Apo 20X/0.8, Plan-Apo 40X/1.4oil, C-Apo 40x/1.2water, Plan-Apo 63X/1.4oil
- Airyscan superresolution detector (32-channel (GaAsP-PMT) that collects a pinhole-plane image at every scan position
- 2x better resolution than classic confocal instruments
- Instrument cost per hour: $57.00
Axio Imager.M2 Motorized Upright Microscope:
- Fluorescence LED Illumination – DAPI, GFP, Cy3, Cy5
- Axiocam 506 color camera and Axiocam HRm
- Automated Multichannel, Z-Stacks, Motorized X-Y Stage for Automated Large Region Tiling
- High NA Objectives: 1.25x, 5x, 10x, 20x, 40x, and 63x with phase and DIC optics
- Apotome.2 Optical Sectioning Slider
- ZEN Blue version 2.3 software
- Instrument Cost per Hour: $28.00
Zeiss Cell Discoverer 7:
- LED lines at 405, 458, 477, 488, 514, 543 and 633 nm
- Petri dishes, chamber slides, multiwell plates, plastic or glass, thin or thick vessel bottoms, low skirt or high skirt plates.
- Objectives include PlanApochromat 5x/0.35, 20x/0.7, 20x/0.95, plus PlanApochromat 50x/1.2 water Auto correction and auto water immersion
- Timed-Series, Z-stacking, and multi-region or large-region Tile Imaging options
- Definite focus, Incubation (Temperature & CO2), and motorized stage
- ZEN Blue software
- Instrument Cost per Hour: $41.00
ImageXpress Pico & BioTek Cytation 7
- Different Sample Holders: Plates, Slides
- Read Method: Live preview imaging with digital confocal, Kinetic, well-area scanning
- Luminescence wavelength range: 300 – 700nm
- Fluorescence wavelength selection: Five channels with images, spectral scanning without imaging
- Absorbance: 200 to 999 nm, tunable in 1 nm increments, available for well scanning
- Temperature control up to 50° C
- Uses CellReporterExpress Software with Windows 10, Gen 5 ver 3.09
- Instrument cost per hour: $28.00
Cryostar NX70 Cryostat
- LCD touch-screen and joystick controls
- User-adjustable LED lighting
- Motorized and manual sectioning
- Vacutome for generating wrinkle-free sections
- Integrated height adjustments for users
- Rapid response temperature control
- Novel knife carrier feed with better sectioning quality
- Optional Cold disinfection for complete surface disinfection
- Instrument cost per hour: $21.00
FEI Transmission Electron Microscope
- High-resolution TEM with top entry stage
- High-Voltage range: 40 to 100kV in steps of 10kV
- Intensity zoom: allows for constant screen brightness at different magnifications
- Intensity limit: prevents electron beam intensity overload on sample
- Magnification: 25 – 200000 x
- Automatic saving of full exposure sequence
- Ultrathin sections generated using Leica UC7 Ultramicrotome
- Instrument cost per hour: $52.00
Ultracut UC7 Ultramicrotome
- High-quality ultra- or semi-thin sections of a specimen for transmission electron microscopy
- Three built-in LED light sources provide outstanding illumination for top light, back light, and specimen transillumination
- Instrument cost per hour: $31.00
Fully Equipped Cell & Tissue Culture Facility
- Biosafety Cabinet
- Incubators
- Cell counter and other support equipment