Biosafety requirements and rules
- Selected rooms in the Image Analysis Laboratory (IAL) have been approved as BL-2 laboratory spaces.
- In order to be able to bring the BL-2 material to the IAL, you MUST list the IAL facility and room number in the investigator’s IBC permit, in Section F, Agent Use and Storage Locations.
- The investigator is required to send a copy of the IBC permit listing the relevant IAL laboratory room and the BL2 organisms to Dr. Robert Burghardt at rburghardt@cvm.tamu.edu before bringing the BL-2 samples.
- All users must follow the rules posted when using the BL-2 labs in the IAL.
- This applies even to those users that do not work with samples requiring an IBC permit.
Procedures for scheduling and use of microscopy services and instrumentation
- Please register in iLabs for scheduling and billing at https://tamu.corefacilities.org/service_center/show_external/4876.
- For the first-time use of:
- optical microscopy technologies, contact Dr. Robert Burghardt at rburghardt@cvm.tamu.edu or Dr. Saugata Mahapatra at saugata@tamu.edu.
- transmission electron (TEM) microscopy, contact Dr. Joseph Szule at jszule@cvm.tamu.edu.
- Microscopes may be checked out by one of the core facility staff for the desired application and operated by trained individuals.
- Each investigator using fluorescence facilities is responsible for the design and analysis of their experiments.
- Core facility staff may provide experimental design and data analysis support based on normal collaborative research agreements.
- Scheduling any fluorescence imaging equipment may be done up to, but not exceeding four weeks in advance.
- A maximum of four hours per day per user during regular business peak hours (8:00 a.m.–5:00 p.m.) may be scheduled in advance.
- If no one else has signed up, the machine may be used until the next scheduled user arrives.
- To avoid being charged for the scheduled use of a microscope, a notice of cancellation must be received during regular business hours on the day prior to an appointment.
- Data should be moved from each instrument to each user’s preferred storage media at the end of each imaging session.
- The IAL is not responsible for data that is left on the instrument for more than one week.
Equipment Checkout
- All new optical and electron microscopes and other major equipment users are required to demonstrate competency instruments they wish to operate.
- Users who have qualified on instruments elsewhere must be instructed and certified for IAL equipment prior to checkout.
- Users must be able to assess the working condition of an instrument, and they will be held responsible for informing IAL staff if an instrument isn’t performing properly.
Operator Responsibilities
- All microscopes are maintained in a ready state by IAL staff, permitting users to sign-up for instruments during business hours.
- Written logs are maintained for each machine, with lamp hours and any other issues recorded.
Billing
- Use of instruments incur user fees, which are posted annually.
- Users MUST have an account number on file prior to using the IAL.
- Separate charges are billed for consumable supplies, technical support, and staff-assisted projects.
- Invoices are prepared monthly.
Safety
- All users of the IAL are responsible for the safe utilization of instrumentation.
- Electrical and chemical hazards, as well as hazards associated with the utilization of high-vacuum instruments, exist.
- No biohazardous material may be brought into the laboratory unless procedures for their use and disposal have been approved and all IAL staff are properly informed of potential hazards.
- A handbook of safety procedures and a Texas A&M University Hazardous Waste Disposal Manual are located at the laboratory’s entrance.
- Users of the laboratory are obligated to use proper safety procedures and are expected to both read and understand the contents of these documents.
- Material Data Safety Sheets are maintained on all chemicals used in the laboratory.
- Contact an IAL staff member for manuals.
Publication Acknowledgment
- Please include the core name and RRID in the acknowledgment section for publications containing data or analysis generated in the facility.
- For example: “The authors acknowledge the assistance of the Image Analysis Laboratory, Texas A&M Veterinary Medicine & Biomedical Sciences. RRID: SCR_022479″
IAL Staff Members
- Robert C. Burghardt, Ph.D. | Director
- Joseph Szule, Ph.D. | Assistant Research Professor
- Saugata Mahapatra, Ph.D. | Associate Research Scientist
- Ernesto Ramos, M.S. | Senior Research Associate
- Igbal Awooda, M.D., M.S. | Research Assistant