The Dysbiosis Index (DI) is a PCR based assay that quantifies the abundances of 7 bacterial groups and total bacteria and summarizes them in one single number .
The DI allows veterinarians to assess whether a dog has a normal or abnormal fecal microbiota composition. This assay has been used in more than 30 clinical studies, and we have established reference intervals based on healthy dogs from various countries worldwide (Table 1).
As a secondary interpretation, based on the abundance of Clostridium hiranonis, the assay can predict normal or abnormal conversion of bile acids in the intestine (i.e., predicts a lack of conversion of primary to secondary bile acids), as the lack of secondary BA is a major contributor to an abnormal microbiota.
|Table 1. Reference intervals|
|Normal abundance||Change observed in dysbiosis|
|Faecalibacterium||3.4 – 8.0||decreased|
|Turicibacter||4.6 – 8.1||decreased|
|Streptococcus||1.9 – 8.0||increased|
|E. coli||0.9 – 8.0||increased|
|Blautia||9.5 – 11.0||decreased|
|Fusobacterium||7.0 – 10.3||decreased|
|C. hiranonis||5.1 – 7.1||decreased|
|Dysbiosis Index||< 0 normal
> 2 dysbiosis
|Data expressed log DNA/gram of feces|